Department of Chemistry, University of Kansas, 1567 Irving Hill Road, Lawrence, KS, 66045, USA.
Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, KS, 66047, USA.
Anal Bioanal Chem. 2020 Sep;412(24):6101-6119. doi: 10.1007/s00216-020-02647-z. Epub 2020 Apr 28.
Microchip electrophoresis with amperometric detection (ME-EC) is a useful tool for the determination of redox active compounds in complex biological samples. In this review, a brief background on the principles of ME-EC is provided, including substrate types, electrode materials, and electrode configurations. Several different detection approaches are described, including dual-channel systems for dual-electrode detection and electrochemistry coupled with fluorescence and chemiluminescence. The application of ME-EC to the determination of catecholamines, adenosine and its metabolites, and reactive nitrogen and oxygen species in microdialysis samples and cell lysates is also detailed. Lastly, approaches for coupling of ME-EC with microdialysis sampling to create separation-based sensors that can be used for near real-time monitoring of drug metabolism and neurotransmitters in freely roaming animals are provided. Graphical abstract.
微芯片电泳安培检测（ME-EC）是一种用于测定复杂生物样品中氧化还原活性化合物的有用工具。在本综述中，简要介绍了 ME-EC 的原理，包括基质类型、电极材料和电极结构。描述了几种不同的检测方法，包括用于双电极检测的双通道系统以及与荧光和化学发光相结合的电化学。还详细介绍了 ME-EC 在微透析样品和细胞裂解物中儿茶酚胺、腺苷及其代谢物以及活性氮和氧物种的测定中的应用。最后，提供了将 ME-EC 与微透析采样耦合的方法，以创建基于分离的传感器，可用于自由游动动物中药物代谢和神经递质的实时监测。
