羟丙基纤维素与海藻糖联合用于人类卵母细胞玻璃化冷冻的补充剂：一项回顾性队列研究
A combination of hydroxypropyl cellulose and trehalose as supplementation for vitrification of human oocytes: a retrospective cohort study.
作者信息
Coello Aila, Campos Pilar, Remohí José, Meseguer Marcos, Cobo Ana
机构信息
Instituto Valenciano de Infertilidad (IVI), University of Valencia, Pl. Policía Local 3, Valencia, Spain.
出版信息
J Assist Reprod Genet. 2016 Mar;33(3):413-421. doi: 10.1007/s10815-015-0633-9. Epub 2016 Jan 11.
PURPOSE
This study aimed to determine whether the new formulation of vitrification solutions containing a combination of hydroxypropyl cellulose (HPC) and trehalose does not affect outcomes in comparison with using conventional solutions made of serum substitute supplement (SSS) and sucrose.
METHODS
Ovum donation cycles were retrospectively compared regarding the solution used for vitrification and warming of human oocytes. The analysis included 218 cycles (N = 2532 oocytes) in the study group (HPC + trehalose) and 214 cycles (N = 2353 oocytes) in the control group (SSS + sucrose).
RESULTS
No statistical differences were found in ovarian stimulation parameters and baseline characteristics of donors and recipients. The survival rate was 91.3% (95% confidence interval (CI) = 89.8-92.9) in the HPC + trehalose group vs. 92.1% (95% CI = 90.4-93.7) in the SSS + sucrose group (NS). The implantation rate (42.8%, 95% CI = 37.7-47.9 vs. 41.2%, 95% CI = 36.0-46.4), clinical pregnancy rate (CPR) per transfer (60.7%, 95% CI = 53.9-67.5 vs. 56.4%, 95% CI = 49.3-63.5), and ongoing pregnancy rate (OPR) per transfer (48.5%, 95% CI = 41.5-55.5 vs. 46.3%, 95% CI = 39.2-53.4) were similar for patients who received either HPC + trehalose-vitrified oocytes or SSS + sucrose-vitrified oocytes. Statistical differences were found when analyzing blastocyst rate both per injected oocyte (30.2%, 95% CI = 28.3-32.1 vs. 24.1%, 95% CI = 22.3-25.9) and per fertilized oocyte (40.8%, 95%CI = 38.5-43.1 vs. 33.2%, 95% CI = 30.8-35.5) (P < 0.0001). Delivery rate was comparable between groups (37.2%, 95% CI = 30.8-46.6 vs. 36.9%, 95% CI = 30.4-43.4; NS).
CONCLUSIONS
Our data demonstrate that HPC and trehalose are suitable and safe substitutes for serum and sucrose. Therefore, the new commercial media can be used efficiently in the vitrification of human oocytes avoiding viral and endotoxin contamination risk.
目的
本研究旨在确定含有羟丙基纤维素（HPC）和海藻糖组合的新型玻璃化溶液配方与使用由血清替代补充剂（SSS）和蔗糖制成的传统溶液相比，是否不会影响结果。
方法
回顾性比较人类卵母细胞玻璃化和复温所用溶液的卵子捐赠周期。分析包括研究组（HPC + 海藻糖）中的218个周期（N = 2532个卵母细胞）和对照组（SSS + 蔗糖）中的214个周期（N = 2353个卵母细胞）。
结果
在卵巢刺激参数以及供体和受体的基线特征方面未发现统计学差异。HPC + 海藻糖组的存活率为91.3%（95%置信区间（CI）= 89.8 - 92.9），而SSS + 蔗糖组为92.1%（95%CI = 90.4 - 93.7）（无统计学差异）。接受HPC + 海藻糖玻璃化卵母细胞或SSS + 蔗糖玻璃化卵母细胞的患者，其植入率（42.8%，95%CI = 37.7 - 47.9对41.2%，95%CI = 36.0 - 46.4）、每次移植的临床妊娠率（CPR）（60.7%，95%CI = 53.9 - 67.5对56.4%，95%CI = 49.3 - 63.5）以及每次移植的持续妊娠率（OPR）（48.5%，95%CI = 41.5 - 55.5对46.3%，95%CI = 39.2 - 53.4）相似。在分析每个注射卵母细胞的囊胚率（30.2%，95%CI = 28.3 - 32.1对24.1%，95%CI = 22.3 - 25.9）和每个受精卵母细胞的囊胚率（40.8%，95%CI = 38.5 - 43.1对33.2%，95%CI = 30.8 - 35.5）时发现有统计学差异（P < 0.0001）。两组的分娩率相当（37.2%，95%CI = 30.8 - 46.6对36.9%，95%CI = 30.4 - 43.4；无统计学差异）。
结论
我们的数据表明，HPC和海藻糖是血清和蔗糖合适且安全的替代品。因此，这种新型商业培养基可有效地用于人类卵母细胞的玻璃化，避免病毒和内毒素污染风险。
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