玻璃化溶液中添加羟丙基纤维素：一项关于供体卵母细胞的前瞻性研究。
Hydroxypropyl cellulose supplementation in vitrification solutions: a prospective study with donor oocytes.
作者信息
Gallardo Miguel, Hebles María, Migueles Beatriz, Dorado Mónica, Aguilera Laura, González Mercedes, Piqueras Paloma, Lucas Alejandro, Montero Lorena, Sánchez-Martín Pascual, Sánchez-Martín Fernando, Risco Ramón
机构信息
Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
Seville Engineering School, University of Seville, Camino de los Descubrimientos s/n, 41092, Seville, Spain.
出版信息
J Assist Reprod Genet. 2017 Mar;34(3):417-422. doi: 10.1007/s10815-016-0841-y. Epub 2016 Dec 27.
PURPOSE
Hydroxypropyl cellulose (HPC), a polysaccharide that forms a viscous gel under low temperatures, is a promising substitute of the blood-derived macromolecules traditionally used in cryopreservation solutions. The performance of a protein-free, fully synthetic set of vitrification and warming solutions was assessed in a matched pair analysis with donor oocytes.
METHODS
A prospective study including 219 donor MII oocytes was carried out, comparing the laboratory outcomes of oocytes vitrified with HPC-based solutions and their fresh counterparts. The primary performance endpoint was the fertilization rate. Secondary parameters assessed were embryo quality on days 2 and 3.
RESULTS
70/73 (95.9%) vitrified MII oocytes exhibited morphologic survival 2 h post-warming, with 49 (70.0%) presented normal fertilization, compared to 105 of 146 (71.9%) MII fresh oocytes. Similar embryo quality was observed in both groups. A total of 18 embryos implanted, out of 38 embryos transferred (47.3%), resulting in 13 newborns.
目的
羟丙基纤维素（HPC）是一种在低温下形成粘性凝胶的多糖，是传统用于冷冻保存溶液的血液衍生大分子的一种有前景的替代品。在与供体卵母细胞的配对分析中评估了一组无蛋白、完全合成的玻璃化和复温溶液的性能。
方法
进行了一项包括219个供体MII期卵母细胞的前瞻性研究，比较了用基于HPC的溶液玻璃化的卵母细胞及其新鲜对应物的实验室结果。主要性能终点是受精率。评估的次要参数是第2天和第3天的胚胎质量。
结果
70/73（95.9%）的玻璃化MII期卵母细胞在复温后2小时表现出形态学存活，其中49个（70.0%）受精正常，相比之下，146个新鲜MII期卵母细胞中有105个（71.9%）受精正常。两组观察到相似的胚胎质量。在38个移植胚胎中，共有18个胚胎着床（47.3%），产下13名新生儿。
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