Maeda H, Saiki I, Ishida H, Kiso M, Hasegawa A, Azuma I
Institute of Immunological Science, Hokkaido University, Sapporo, Japan.
Vaccine. 1989 Jun;7(3):275-81. doi: 10.1016/0264-410x(89)90243-0.
We investigated the effects of the active principle of lipopolysaccharide (LPS), synthetic lipid A (compound 506), and of its related compounds GLA-60, -59 and -27, on murine macrophage activation and cytokine induction. GLA-60, which is devoid of endotoxic activity, showed interleukin-1 (IL-1)-inducing activity and activation of murine macrophages comparable to those of LPS or compound 506. The biological activities of six conjugates of GLA-60 with MDP derivatives GMD-323 to -328 were investigated in this study. All the GMD compounds except GMD-323 showed potent inducing activities for IL-1 and tumoricidal macrophages, especially GMD-324 and -326, which exhibited much higher activity than GLA-60. However, TNF- and CSF-inducing activities of these conjugates were lower than those of GLA-60. IL-1-inducing activity of the mixture of MDP derivative (GMD-267) and GLA-60 was higher than that of the conjugates (GMD-324) or that of GLA-60 and GMD-267 alone.
我们研究了脂多糖（LPS）的活性成分、合成类脂A（化合物506）及其相关化合物GLA - 60、- 59和- 27对小鼠巨噬细胞激活和细胞因子诱导的影响。无内毒素活性的GLA - 60表现出与LPS或化合物506相当的诱导白细胞介素-1（IL - 1）的活性以及激活小鼠巨噬细胞的能力。本研究考察了GLA - 60与MDP衍生物GMD - 323至- 328的六种缀合物的生物学活性。除GMD - 323外，所有GMD化合物均对IL - 1和杀肿瘤巨噬细胞表现出强效诱导活性，尤其是GMD - 324和- 326，其活性远高于GLA - 60。然而，这些缀合物诱导肿瘤坏死因子（TNF）和集落刺激因子（CSF）的活性低于GLA - 60。MDP衍生物（GMD - 267）与GLA - 60混合物诱导IL - 1的活性高于缀合物（GMD - 324）或单独的GLA - 60和GMD - 267。
