Saito-Taki T, Nakano M, Kiso M, Hasegawa A
Microbiol Immunol. 1985;29(11):1111-20. doi: 10.1111/j.1348-0421.1985.tb00901.x.
Direct stimulations of murine B lymphocytes with synthetic lipid A analogs and synthetic muramyl dipeptide (MDP) derivatives were studied using a limiting dilution assay system. Synthetic lipid A analogs, GLA-27 and GLA-40, when conjugated with bovine serum albumin (BSA) had the ability to induce B cell clonal expansion of a single B cell from the spleen or bone-marrow. Their activities were almost the same as those of naturally obtained lipid A, but were lower than that of bacterial lipopolysaccharide (LPS). Addition of dextran sulfate (DXS) enhanced the effect of lipid A analogs. In contrast, synthetic MDP and its derivatives, although they had many biological and immunological activities in experimental animals, could not stimulate a single B cell to induce clonal expansion regardless of the presence or absence of DXS. These results suggested that lipid A analogs can directly cause the proliferation of B cells, but MDPs can not.
使用有限稀释分析系统研究了用合成脂多糖A类似物和合成胞壁酰二肽（MDP）衍生物对小鼠B淋巴细胞的直接刺激作用。合成脂多糖A类似物GLA-27和GLA-40与牛血清白蛋白（BSA）偶联时，能够诱导来自脾脏或骨髓的单个B细胞发生B细胞克隆扩增。它们的活性与天然获得的脂多糖A几乎相同，但低于细菌脂多糖（LPS）。添加硫酸葡聚糖（DXS）可增强脂多糖A类似物的作用。相比之下，合成MDP及其衍生物虽然在实验动物中具有许多生物学和免疫学活性，但无论是否存在DXS，都不能刺激单个B细胞诱导克隆扩增。这些结果表明，脂多糖A类似物可直接导致B细胞增殖，而MDP则不能。
