Wang Kai, Zheng Wenting, Pan Yiwa, Ma Shaoyang, Guan Yong, Liu Ruming, Zhu Meifeng, Zhou Xin, Zhang Jun, Zhao Qiang, Zhu Yan, Wang Lianyong, Kong Deling
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin, 300071, China.
Department of Pathology, St. Jude Children Research Hospital, Memphis, TN, 38105, USA.
Macromol Biosci. 2016 Apr;16(4):608-18. doi: 10.1002/mabi.201500355. Epub 2016 Jan 12.
In this study, a three layered poly (ε-caprolactone) (PCL) graft (tPCL) was fabricated by electrospinning PCL and electrospraying poly (ethylene oxide) (PEO), which has a thin dense inner layer, a loose middle layer, and a dense outer layer. Regular PCL grafts (rPCL) with only a dense layer were used as control. In vivo evaluation was performed in rabbit carotid artery. Enhanced cell infiltration, rapid regeneration of endothelium and smooth muscle layers, and increased elastin deposition were observed within the tPCL graft wall. After 3 months, tPCL grafts showed faster PCL degradation than the rPCL grafts. Infiltrated macrophages in the tPCL grafts secreted higher level of monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) which enhanced vascular regeneration. In conclusion, the tPCL graft may be a useful vascular prosthesis and worth for further investigation.
在本研究中，通过静电纺丝聚己内酯（PCL）和静电喷涂聚环氧乙烷（PEO）制备了一种三层聚己内酯（PCL）移植物（tPCL），其具有薄而致密的内层、疏松的中层和致密的外层。仅具有致密层的常规PCL移植物（rPCL）用作对照。在兔颈动脉中进行了体内评估。在tPCL移植物壁内观察到细胞浸润增强、内皮和平滑肌层快速再生以及弹性蛋白沉积增加。3个月后，tPCL移植物显示出比rPCL移植物更快的PCL降解。tPCL移植物中浸润的巨噬细胞分泌更高水平的单核细胞趋化蛋白-1（MCP-1）和血管内皮生长因子（VEGF），这增强了血管再生。总之，tPCL移植物可能是一种有用的血管假体，值得进一步研究。
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