Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
Institute of Translational Pharmacology (IFT)-CNR, L'Aquila, Italy.
J Cell Physiol. 2016 Sep;231(9):2014-25. doi: 10.1002/jcp.25310. Epub 2016 Jan 28.
Extremely low frequency magnetic fields (ELF-MF) are common environmental agents that are suspected to promote later stages of tumorigenesis, especially in brain-derived malignancies. Even though ELF magnetic fields have been previously linked to increased proliferation in neuroblastoma cells, no previous work has studied whether ELF-MF exposure may change key biomolecular features, such as anti-glycative defence and energy re-programming, both of which are currently considered as crucial factors involved in the phenotype and progression of many malignancies. Our study investigated whether the hyperproliferation that is induced in SH-SY5Y human neuroblastoma cells by a 50 Hz, 1 mT ELF magnetic field is supported by an improved defense towards methylglyoxal (MG), which is an endogenous cancer-static and glycating α-oxoaldehyde, and by rewiring of energy metabolism. Our findings show that not only the ELF magnetic field interfered with the biology of neuron-derived malignant cells, by de-differentiating further the cellular phenotype and by increasing the proliferative activity, but also triggered cytoprotective mechanisms through the enhancement of the defense against MG, along with a more efficient management of metabolic energy, presumably to support the rapid cell outgrowth. Intriguingly, we also revealed that the MF-induced bioeffects took place after an initial imbalance of the cellular homeostasis, which most likely created a transient unstable milieu. The biochemical pathways and molecular targets revealed in this research could be exploited for future approaches aimed at limiting or suppressing the deleterious effects of ELF magnetic fields. J. Cell. Physiol. 231: 2014-2025, 2016. © 2016 Wiley Periodicals, Inc.
极低频磁场（ELF-MF）是常见的环境因子，据推测其会促进肿瘤发生的后期阶段，尤其是在脑源性恶性肿瘤中。尽管先前已经证明 ELF 磁场会增加神经母细胞瘤细胞的增殖，但尚无研究探讨 ELF-MF 暴露是否会改变关键生物分子特征，如抗糖化防御和能量重编程，这两者目前都被认为是许多恶性肿瘤表型和进展的关键因素。我们的研究调查了 50 Hz、1 mT 的 ELF 磁场是否会通过增强对甲基乙二醛（MG）的防御来支持 SH-SY5Y 人神经母细胞瘤细胞的过度增殖，MG 是一种内源性的癌症抑制物和糖化 α-氧代醛，以及通过能量代谢的重新布线。我们的研究结果表明，ELF 磁场不仅通过进一步去分化细胞表型和增加增殖活性来干扰神经元衍生的恶性细胞的生物学特性，而且还通过增强对 MG 的防御以及更有效地管理代谢能量来触发细胞保护机制，可能是为了支持快速的细胞生长。有趣的是，我们还发现，MF 诱导的生物效应发生在细胞内稳态的初始失衡之后，这很可能造成了短暂的不稳定环境。本研究揭示的生化途径和分子靶标可用于未来的方法，旨在限制或抑制 ELF 磁场的有害影响。J. Cell. Physiol. 231: 2014-2025, 2016. © 2016 Wiley Periodicals, Inc.
