Prause J U, Jensen O A, Paschides K, Støvhase A, Vangsted P
Eye Pathology Institute, University of Copenhagen, Denmark.
J Autoimmun. 1989 Aug;2(4):495-500. doi: 10.1016/0896-8411(89)90181-9.
Formalin-fixed biopsies of conjunctival tissue from the lower fornix of 10 healthy persons and of 10 patients with primary Sjögren's syndrome (primary SS) were examined by light microscopy. Deparaffinized, rehydrated sections of 5 microns were incubated overnight, each with one of 15 different biotinylated lectins. Bound lectins were visualized using avidin-HRP-substrate complexes. Five lectins did not bind to conjunctival cells, and further six lectins bound in an unspecific manner to conjunctival cells of both healthy persons and of primary SS patients. Jacalin lectin bound selectively to goblet cells of all specimens. Peanut agglutinin (PNA) and wheat germ agglutinin (WGA) bound significantly stronger to basal conjunctival epithelial cells of patients with primary SS. This binding pattern may be of diagnostic value.
对10名健康人和10名原发性干燥综合征（原发性SS）患者下穹窿结膜组织的福尔马林固定活检标本进行了光学显微镜检查。将5微米的脱石蜡、复水切片与15种不同的生物素化凝集素之一孵育过夜。使用抗生物素蛋白-辣根过氧化物酶-底物复合物观察结合的凝集素。5种凝集素未与结膜细胞结合，另外6种凝集素以非特异性方式与健康人和原发性SS患者的结膜细胞结合。红豆凝集素选择性地与所有标本的杯状细胞结合。花生凝集素（PNA）和麦胚凝集素（WGA）与原发性SS患者结膜基底上皮细胞的结合明显更强。这种结合模式可能具有诊断价值。
