Nairn R S, McIntyre B W, Richie E R, Allison J P
Department of Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville.
Mol Carcinog. 1989;2(3):126-30. doi: 10.1002/mc.2940020304.
A role for specific recombination events at the env region of endogenous murine leukemia virus (MuLV) sequences in radiation carcinogenesis in C57BL mice has been suggested by a number of studies. We characterized env-related cell surface antigens from a primary, x-ray--induced, and several transplanted C57BL/6 thymomas of viral and radiation etiologies by immunoprecipitation and two-dimensional peptide mapping. DNA from lymphoma cells was also analyzed by Southern blotting for evidence of mink cell focus-forming (MCF) type env recombination events. Although gp70 molecules with novel structural determinants were found on all transplanted lymphomas examined, expression of novel env antigens was variable among these lymphomas, and there was a lack of correlation of characteristic MCF-type env recombination events in endogenous retrovirus DNA sequences with novel env antigens on lymphoma cell surfaces. Neither novel gp70 antigens nor MCF-type env provirus recombinant structures were consistent features of the C57BL/6 thymomas of radiation etiology examined in this study, even though MCF-type env recombination events have been suggested as etiologically significant in MuLV-mediated lymphomagenesis in both RadLV and x-ray--induced tumors in C57BL/6 mice.
多项研究表明，内源性鼠白血病病毒（MuLV）序列的env区域发生的特定重组事件在C57BL小鼠的辐射致癌过程中发挥了作用。我们通过免疫沉淀和二维肽图分析，对源自原发性、X射线诱导的以及几种病毒和辐射病因的移植性C57BL/6胸腺瘤的env相关细胞表面抗原进行了表征。还通过Southern印迹法分析了淋巴瘤细胞的DNA，以寻找貂细胞集落形成（MCF）型env重组事件的证据。尽管在所检查的所有移植性淋巴瘤中均发现了具有新结构决定簇的gp70分子，但这些淋巴瘤中新型env抗原的表达存在差异，并且内源性逆转录病毒DNA序列中特征性的MCF型env重组事件与淋巴瘤细胞表面的新型env抗原之间缺乏相关性。即使在RadLV和X射线诱导的C57BL/6小鼠肿瘤中，MCF型env重组事件在MuLV介导的淋巴瘤发生中被认为具有病因学意义，但在本研究中检查的辐射病因的C57BL/6胸腺瘤中，新型gp70抗原和MCF型env前病毒重组结构均不是一致的特征。
