Nyyssönen K, Parviainen M T
Department of Clinical Chemistry, University of Kuopio, Finland.
Crit Rev Clin Lab Sci. 1989;27(3):211-36. doi: 10.3109/10408368909105714.
In this review the methods used for analysis of plasma catecholamines in clinical chemical laboratories are discussed. The physiology of catecholamines as well as their measuring indications are discussed, together with concise evaluation of the methods most commonly used, namely indirect radioenzymatic assays or direct determinations by high-performance liquid chromatography combined with either electrochemical or fluorometric detection. The main advantage of radioenzymatic assay is its sensitivity and thus the need for only a small sample. Liquid chromatographic methods in general are less tedious, relatively rapid, and cheap, and omit the use of radionuclides. Both of these methods, however, are subject to a number of analytical errors, which can only be avoided by proper development of methods and skilled use of these methods. Little routine work is done using either radioimmunoassay or gas-chromatography.
在本综述中，讨论了临床化学实验室中用于分析血浆儿茶酚胺的方法。文中讨论了儿茶酚胺的生理学及其测量指征，并对最常用的方法进行了简要评估，即间接放射酶法或高效液相色谱结合电化学或荧光检测的直接测定法。放射酶法的主要优点是其灵敏度高，因此只需要少量样本。一般来说，液相色谱法操作不太繁琐，相对快速且成本较低，并且无需使用放射性核素。然而，这两种方法都存在一些分析误差，只有通过适当开发方法和熟练使用这些方法才能避免。放射免疫分析或气相色谱法的常规应用较少。
