Peaston Robert T, Weinkove Cyril
Department of Clinical Biochemistry, Freeman Hospital, Newcastle Upon Tyne Hospitals NHS Trust, Newcastle Upon Tyne NE7 7DN, UK.
Ann Clin Biochem. 2004 Jan;41(Pt 1):17-38. doi: 10.1258/000456304322664663.
Analysis of the low levels of catecholamines and metabolites in tissue and biological fluids has necessitated the use of highly sensitive analytical techniques. Earlier procedures utilizing radioenzymatic and immunological assays, gas chromatography or fluorimetry have generally been superseded by highly sensitive and selective chromatographic methods utilizing electrochemical or fluorimetric detection. The development of high-performance liquid chromatography (HPLC) methods for the measurement of plasma metadrenalines and the combination of HPLC with tandem mass spectrometry provides additional procedures with minimum interference from drugs and drug metabolites. This review summarizes the methodology currently available for the measurement of catecholamines and metabolites in plasma and urine, the influence of sample collection protocols and the clinical application of the methods for the biochemical detection of catecholamine-secreting tumours.
对组织和生物体液中低水平儿茶酚胺及其代谢物的分析需要使用高灵敏度的分析技术。早期使用放射酶法和免疫测定法、气相色谱法或荧光测定法的程序，通常已被采用电化学或荧光检测的高灵敏度和选择性色谱方法所取代。用于测量血浆间甲肾上腺素的高效液相色谱（HPLC）方法的开发以及HPLC与串联质谱联用，提供了受药物和药物代谢物干扰最小的额外程序。本综述总结了目前可用于测量血浆和尿液中儿茶酚胺及其代谢物的方法、样本采集方案的影响以及这些方法在儿茶酚胺分泌肿瘤生化检测中的临床应用。
