De Jonge M W, De Leij L, Mesander G, Deenen G J, Opstelten D, Kroese F G, van Imhoff G W, Nieuwenhuis P, The T H
Department of Clinical Immunology, University of Groningen, The Netherlands.
Cancer Res. 1989 Oct 15;49(20):5682-8.
A late pre-B-cell leukemia model in the rat, the LAMA tumor, is described. A mouse monoclonal antibody (HIS30) was developed against LAMA cells. HIS30 reacts with a membrane antigen in tumor tissue, whereas its reactivity with normal tissues is limited to the zona glomerulosa of the adrenal cortex and to the adrenal medulla. HIS30 was used for both the immunohistological detection of tumor cells in tissue sections and the immunolocalization of tumor cells in vivo. To enable in vitro studies with the LAMA model, an in vitro growing cell line (LAMA-K1) was established from the LAMA tumor. LAMA-K1 is immunophenotypically similar to the original tumor. Two tumor transplantation models were characterized. In the first model LAMA was implanted s.c., and local tumor growth occurred at the injection site, which was then followed by lymphatogenic and subsequently hematogenic tumor spread. In the second model i.v. transplantation caused direct hematogenic tumor dissemination. In both models early dissemination was especially prominent to the bone marrow, spleen, and liver. Later in the disease most visceral organs became involved, and partial paralysis of the animal was observed in the end stage of the disease. In combination with HIS30, the LAMA pre-B-cell tumor offers a model for both the investigation of in vivo transplanted tumor cells and for the in vivo detection of tumor cells by HIS30 in LAMA tumor-bearing rats.
本文描述了一种大鼠晚期前B细胞白血病模型，即LAMA肿瘤。制备了一种针对LAMA细胞的小鼠单克隆抗体（HIS30）。HIS30与肿瘤组织中的一种膜抗原发生反应，而其与正常组织的反应仅限于肾上腺皮质的球状带和肾上腺髓质。HIS30用于组织切片中肿瘤细胞的免疫组织化学检测以及体内肿瘤细胞的免疫定位。为了能够使用LAMA模型进行体外研究，从LAMA肿瘤中建立了一种体外生长的细胞系（LAMA-K1）。LAMA-K1在免疫表型上与原始肿瘤相似。对两种肿瘤移植模型进行了表征。在第一个模型中，将LAMA皮下植入，在注射部位出现局部肿瘤生长，随后发生淋巴源性和随后的血源性肿瘤扩散。在第二个模型中，静脉内移植导致直接的血源性肿瘤播散。在这两种模型中，早期播散在骨髓、脾脏和肝脏中尤为明显。在疾病后期，大多数内脏器官都受到影响，在疾病末期观察到动物部分瘫痪。与HIS30相结合，LAMA前B细胞肿瘤为研究体内移植的肿瘤细胞以及通过HIS30在荷LAMA肿瘤大鼠体内检测肿瘤细胞提供了一个模型。
