Kubonová K, Jancula L
Cesk Epidemiol Mikrobiol Imunol. 1989 Sep;38(5):286-91.
During the period between March 1987 and July 1988 the authors examined for the presence of Campylobacter pylori bioptic specimens from the gastroduodenum from 410 patients. In the above group they diagnosed by microscopic examination C. pylori in 257 patients (mean 63%). From the whole group 282 patients (100%) were examined concurrently by the microscopic method and by cultivation. This number comprised 195 (68.9%) who were positive on microscopic examination and 110 (42.2%) who were positive on cultivation. A microaerophil medium was obtained by using Gas Pak H2 + CO2 envelopes (Oxoid) and envelopes generating gases H2 + CO2 (JZD Dukla Drahany) without a catalyst and a gas mixture of 85% N2, 10% CO2 and 5% O2. The best detection rate was obtained in basic medium for blood agar no. 4 (Imuna) with 7% ram blood in a defined gas mixture (92%). When investigating the survival of C. pylori in specimens kept in 20% glucose in a refrigerator for 20 or more hours after collection of the sample, the microbe was isolated (from 27 microscopically positive samples) from 19 patients, i.e. 51.2%.
在1987年3月至1988年7月期间，作者对410例患者胃十二指肠的活检标本进行了幽门螺杆菌检测。在上述组中，通过显微镜检查诊断出257例患者（平均63%）存在幽门螺杆菌。对整个组中的282例患者（100%）同时采用显微镜检查法和培养法进行检测。这其中包括显微镜检查呈阳性的195例（68.9%）和培养呈阳性的110例（42.2%）。使用Gas Pak H2 + CO2封套（Oxoid）和不使用催化剂的H2 + CO2产气封套（JZD Dukla Drahany）以及85% N2、10% CO2和5% O2的气体混合物获得微需氧培养基。在含有7%兔血的4号基础血琼脂培养基（Imuna）中，在特定气体混合物（92%）条件下获得了最佳检测率。在对采集样本后置于冰箱中20%葡萄糖溶液中保存20小时及以上的标本中的幽门螺杆菌存活情况进行调查时，从19例患者（即51.2%）中分离出该微生物（从27例显微镜检查呈阳性的样本中）。
