Chinery S A, Hinchliffe E
Delta Biotechnology Ltd., Nottingham, GB.
Curr Genet. 1989 Jul;16(1):21-5. doi: 10.1007/BF00411079.
We have constructed a set of hybrid yeast Escherichia coli vectors which utilise the site specific recombination function of the Saccharomyces cerevisiae 2 microns plasmid to completely eliminate the bacterial moiety upon introduction into yeast. A number of these plasmids have been shown to exhibit high inheritable stability in both laboratory and industrial strains during non-selective growth. These plasmids are beneficial for the genetic modification of industrial yeast, particularly those used in the production of food and beverages, and are of benefit in the study of plasmid maintenance and heterologous gene expression.
我们构建了一组杂交酵母-大肠杆菌载体，这些载体利用酿酒酵母2微米质粒的位点特异性重组功能，在导入酵母后完全消除细菌部分。已证明其中一些质粒在非选择性生长期间在实验室菌株和工业菌株中均表现出高遗传稳定性。这些质粒有利于工业酵母的基因改造，特别是用于食品和饮料生产的酵母，并且在质粒维持和异源基因表达的研究中也有益处。
