Tang Shu-Lun, Bubner Patricia, Bauer Stefan, Somerville Chris R
Energy Biosciences Institute, University of California Berkeley, 2151 Berkeley Way MC 5230, Berkeley, CA 94720, USA.
Energy Biosciences Institute, University of California Berkeley, 2151 Berkeley Way MC 5230, Berkeley, CA 94720, USA
Glycobiology. 2016 Jun;26(6):670-7. doi: 10.1093/glycob/cww004. Epub 2016 Jan 13.
We describe here the composition of the O-linked glycans on the Neurospora crassa cellobiohydrolase I (CBHI), which accounts for approximately 40% of the protein secreted by cells growing in the presence of cellulose. CBHI is O-glycosylated with six types of linear, and three types of branched, O-glycans containing approximately equal amounts of mannose and galactose. In addition to the classic fungal O-glycans with reducing end mannoses, we also identified reducing end galactoses which suggest the existence of a protein-O-galactosyltransferase in N. crassa Because of the excellent genetic resources available for N. crassa, the knowledge of the CBHI O-glycans may enable the future evaluation of the role of O-glycosylation on cellulase function and the development of directed O-glycan/cellulase engineering.
我们在此描述粗糙脉孢菌纤维二糖水解酶I（CBHI）上O-连接聚糖的组成，该酶占在纤维素存在下生长的细胞分泌蛋白的约40%。CBHI通过六种线性和三种分支的O-聚糖进行O-糖基化，这些聚糖含有大致等量的甘露糖和半乳糖。除了具有还原端甘露糖的经典真菌O-聚糖外，我们还鉴定出了还原端半乳糖，这表明粗糙脉孢菌中存在一种蛋白质-O-半乳糖基转移酶。由于粗糙脉孢菌有出色的遗传资源，对CBHI O-聚糖的了解可能有助于未来评估O-糖基化对纤维素酶功能的作用以及定向O-聚糖/纤维素酶工程的发展。
