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一种新型的二元UNC-101/AP-1 μ1结合信号介导秀丽隐杆线虫中KVS-4/Kv2.1的树突状分布。

A novel bipartite UNC-101/AP-1 μ1 binding signal mediates KVS-4/Kv2.1 somatodendritic distribution in Caenorhabditis elegans.

作者信息

Zhou Xin, Zeng Jia, Ouyang Chenxi, Luo Qianyun, Yu Miao, Yang Zhenrong, Wang Hui, Shen Kang, Shi Anbing

机构信息

Department of Medical Genetics, School of Basic Medicine and the Collaborative Innovation Center for Brain Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.

出版信息

FEBS Lett. 2016 Jan;590(1):76-92. doi: 10.1002/1873-3468.12043. Epub 2015 Dec 30.

Abstract

Potassium channels such as Kv2.1 are targeted to specific subcellular compartments to fulfill various functions. However, the mechanisms for their localization are poorly understood. Here, we show that KVS-4/Kv2.1 somatodendritic localization in Caenorhabditis elegansDA9 neuron requires UNC-101(AP-1 μ subunit). We define a bipartite sorting signal within KVS-4 consisting of a C-terminal EQMIL and N-terminal WNIIE motifs. The bipartite signal is sufficient to target nonpolarized transmembrane protein MIG-13 into DA9 somatodendritic compartments. Furthermore, we found that AP-1 interacts with the bipartite signal through UNC-101/AP-1 μ N-terminal predicted Longin-like domain. Our results provide new insight into the mechanisms of Kv2.1 post-Golgi sorting and targeting.

摘要

诸如Kv2.1这样的钾通道定位于特定的亚细胞区室以履行各种功能。然而,其定位机制却知之甚少。在此,我们表明秀丽隐杆线虫DA9神经元中KVS-4/Kv2.1的胞体树突定位需要UNC-101(衔接蛋白1μ亚基)。我们在KVS-4内定义了一个由C端EQMIL和N端WNIIE基序组成的双分型分选信号。该双分型信号足以将非极化跨膜蛋白MIG-13靶向到DA9胞体树突区室。此外,我们发现衔接蛋白1通过UNC-101/衔接蛋白1μ N端预测的类朗金结构域与双分型信号相互作用。我们的结果为Kv2.1高尔基体后分选和靶向机制提供了新的见解。

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