Rajkowski K M, Hanquez C, Bouzoumou A, Cittanova N
U.A. 586 du CNRS, Faculté de Médecine, Paris, France.
Clin Chim Acta. 1989 Aug 15;183(2):197-206. doi: 10.1016/0009-8981(89)90335-5.
An enzyme immunoassay for plasma testosterone was developed based on competition between an immobilised testosterone-casein conjugate and the analyte for polyclonal anti-testosterone immunoglobulins, followed by the use of enzyme-labelled second antibodies to determine the degree of competition. The quantity of immobilised testosterone-casein conjugate was optimised so that the lower affinity anti-testosterone antibody populations present did not affect the assay. The assay standard curve covered a range of 11-300 fmol/well. Testosterone levels in small amounts of male and female plasma could be assayed with good reproducibility and correlated well with results obtained by radioimmunoassay. By comparison with an analogous assay using monoclonal antibodies it appears that, given that the assay sensitivity is the most important criterion for choice, the use a polyclonal antiserum with this type of reactive antibody selection is preferable to the use of monoclonal antibodies.
基于固定化睾酮 - 酪蛋白偶联物与分析物竞争多克隆抗睾酮免疫球蛋白，随后使用酶标记二抗来确定竞争程度，开发了一种用于血浆睾酮的酶免疫测定法。优化了固定化睾酮 - 酪蛋白偶联物的量，以使存在的低亲和力抗睾酮抗体群体不影响测定。该测定标准曲线覆盖范围为11 - 300 fmol/孔。少量男性和女性血浆中的睾酮水平可以进行测定，具有良好的重现性，并且与放射免疫测定获得的结果相关性良好。与使用单克隆抗体的类似测定法相比，鉴于测定灵敏度是选择的最重要标准，使用具有这种类型反应性抗体选择的多克隆抗血清比使用单克隆抗体更可取。
