Hanquez C, Urios P, Desfosses B, Samake H, Lince E, Rajkowski K M, Cittanova N
Clin Chim Acta. 1987 Apr 15;164(1):71-82. doi: 10.1016/0009-8981(87)90108-2.
The development of non-competitive microtitre plate enzyme-linked immunosorbent assays for steroids was investigated using immobilised steroid as immunosorbent and enzyme-labelled second antibodies. An assay for testosterone with polyclonal anti-testosterone immunoglobulins was optimised with respect to a number of parameters but remained unsatisfactory for clinical assays. The results were applied to developing an aldosterone assay using monoclonal anti-aldosterone immunoglobulins. The latter method was used for the determination of urinary aldosterone and the results are compared with those obtained by a classical radioimmunoassay. Problems concerned with the use of sulphur-containing proteins and with the presence of low affinity antibodies in polyclonal preparations are discussed.
研究了以固定化甾体作为免疫吸附剂和酶标记二抗，开发用于甾体的非竞争性微量滴定板酶联免疫吸附测定法。使用多克隆抗睾酮免疫球蛋白对睾酮测定法进行了多项参数优化，但用于临床测定仍不尽人意。将结果应用于开发使用单克隆抗醛固酮免疫球蛋白的醛固酮测定法。后一种方法用于测定尿醛固酮，并将结果与经典放射免疫测定法获得的结果进行比较。讨论了与含硫蛋白质的使用以及多克隆制剂中低亲和力抗体的存在有关的问题。
