Kern Tobias, Fischer Martin A, Deppenmeier Uwe, Schmitz Ruth A, Rother Michael
Institut für Mikrobiologie, Technische Universität Dresden, 01062 Dresden, Germany.
Institut für Allgemeine Mikrobiologie, Universität Kiel, 24118 Kiel, Germany.
Int J Syst Evol Microbiol. 2016 Mar;66(3):1533-1538. doi: 10.1099/ijsem.0.000894. Epub 2016 Jan 12.
A novel, strictly anaerobic, methanogenic archaeon, strain E03.2, was isolated from a full-scale biogas plant in Germany. Cells were non-motile sarcina-like cocci, occurring in aggregates. Strain E03.2 grew autotrophically on H plus CO, and additionally cells could utilize acetate, methanol, moni-, di- and trimethylamine as carbon and energy sources; however, growth or methanogenesis on formate was not observed. Yeast extract and vitamins stimulated growth but were not mandatory. The optimal growth temperature of strain E03.2 was approximately 45 °C; maximal growth rates were obtained at about pH 7.0 in the presence of approximately 6.8 mM NaCl. The DNA G+C content of strain E03.2 was 41.3 mol%. Phylogenetic analyses based on 16S rRNA gene and sequences placed strain E03.2 within the genus . Based on 16S rRNA gene sequence similarity strain E03.2 was related to seven different species of the genus , but most closely related to TM-1. Phenotypic, physiological and genomic characteristics indicated that strain E03.2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is E03.2 ( = DSM 100822 = JCM 30921).
从德国一座全规模沼气厂中分离出了一种新型严格厌氧产甲烷古菌,菌株E03.2。细胞为无运动性的八叠球菌样球菌,呈聚集体形式存在。菌株E03.2能利用H₂和CO₂自养生长,此外细胞还可利用乙酸盐、甲醇、一甲胺、二甲胺和三甲胺作为碳源和能源;然而,未观察到其利用甲酸盐生长或产甲烷。酵母提取物和维生素可刺激生长,但并非必需。菌株E03.2的最适生长温度约为45°C;在约6.8 mM NaCl存在下,于pH约7.0时可获得最大生长速率。菌株E03.2的DNA G+C含量为41.3 mol%。基于16S rRNA基因序列的系统发育分析将菌株E03.2归入某属。基于16S rRNA基因序列相似性,菌株E03.2与该属的七个不同物种相关,但与TM-1关系最为密切。表型、生理和基因组特征表明菌株E03.2代表该属的一个新物种,为此提出新物种名称sp. nov.。模式菌株为E03.2(=DSM 100822 =JCM 30921)。