Favale Nicoletta, Costa Stefania, Summa Daniela, Sabbioni Silvia, Mamolini Elisabetta, Tamburini Elena, Scapoli Chiara
Department of Life Sciences and Biotechnology - Section of Biology and Evolution, University of Ferrara, Italy.
Department of Environmental Sciences and Prevention, University of Ferrara, Ferrara, Italy.
Curr Res Microb Sci. 2025 Mar 31;8:100383. doi: 10.1016/j.crmicr.2025.100383. eCollection 2025.
This study analysed the effect of the different lignocellulose composition of two crop substrates on the structure and dynamics of bacterial communities during anaerobic digestion (AD) processes for biogas production. To this end, cereal grains and grape pomace biomasses were analysed in parallel in an experimental AD bench-scale system to define and compare their metagenomic profiles for different experimental time intervals. The bacterial community structure and dynamics during the AD process were detected and characterised using high-resolution whole metagenomic shotgun analyses. Statistical evaluation identified 15 strains as specific to two substrates. Some strains, like , and sp. MB1 in cereal grains, and and in grape pomace, were never before detected in biogas reactors. The presence of bacteria such as sp. and , which degrade lipids and protein-rich substrates, along with sp. and 1109, which tolerate high hydrogen pressures and ammonia concentrations, suggests a complex syntrophic community in lignin-cellulose-enriched substrates. This finding could help develop new strategies for the production of a tailor-made microbial consortium to be inoculated from the beginning of the digestion process of specific lignocellulosic biomass.
本研究分析了两种作物基质不同的木质纤维素组成对厌氧消化（AD）沼气生产过程中细菌群落结构和动态的影响。为此，在一个实验性AD实验室规模系统中对谷物和葡萄渣生物质进行了平行分析，以确定并比较它们在不同实验时间间隔下的宏基因组图谱。利用高分辨率全宏基因组鸟枪法分析检测并表征了AD过程中的细菌群落结构和动态。统计评估确定了15种菌株是两种基质特有的。一些菌株，如谷物中的 、 和MB1菌属，以及葡萄渣中的 和 ，此前从未在沼气反应器中检测到。诸如能降解富含脂质和蛋白质底物的 菌属和 菌，以及能耐受高氢气压力和氨浓度的 菌属和1109菌的存在，表明在富含木质素 - 纤维素的底物中存在复杂的互营群落。这一发现有助于开发新策略，以生产特定木质纤维素生物质消化过程开始时接种的定制微生物群落。
