Biltoft-Jensen Anja, Damsgaard Camilla T, Andersen Elisabeth W, Ygil Karin H, Andersen Rikke, Ege Majken, Christensen Tue, Thorsen Anne-Vibeke, Tetens Inge, Wu Huaxing, Landberg Rikard
Division of Nutrition, National Food Institute, Technical University of Denmark, Søborg, Denmark;
Department of Nutrition, Exercise and Sports, Faculty of Science, University of Copenhagen, Copenhagen, Denmark;
J Nutr. 2016 Feb;146(2):377-83. doi: 10.3945/jn.115.222620. Epub 2016 Jan 13.
Whole-grain (WG) intake is important for human health, but accurate intake estimation is challenging. Use of a biomarker for WG intake provides a possible way to validate dietary assessment methods.
Our aim was to validate WG intake from 2 diets reported by children, using plasma alkylresorcinol (AR) concentrations, and to investigate the 3-mo reproducibility of AR concentrations and reported WG intake.
AR concentrations were analyzed in fasting blood plasma samples, and WG intake was estimated in a 7-d web-based diary by 750 participants aged 8-11 y in a 2 school meal × 3 mo crossover trial. Reported WG intake and plasma AR concentrations were compared when children ate their usual bread-based lunch (UBL) and when served a hot lunch meal (HLM). Correlations and cross-classification were used to rank subjects according to intake. The intraclass correlation coefficients (ICCs) between subjects' measurements at baseline and after the UBL were used to assess reproducibility.
Correlations between reported WG wheat + rye intake and plasma AR were 0.40 and 0.37 (P < 0.001) for the UBL and the HLM diets, and 78% and 77% were classified in the same or adjacent quartiles for the UBL and HLM diets, respectively. The ICC over 3 mo was 0.47 (95% CI: 0.38, 0.55) for plasma total ARs and 0.64 (95% CI: 0.58, 0.70) for reported WG intake. Correlations were higher when using the AR C17:0 homolog as a biomarker, reflecting rye intake instead of plasma total ARs [UBL: r = 0.47; HLM: r = 0.43, P < 0.001; ICC = 0.51 (95% CI: 0.43, 0.59)].
Self-reported WG wheat + rye intake among children showed moderate correlations with plasma AR concentrations. Substantial intraindividual variation was found in WG intake and plasma AR concentrations. The AR homolog C17:0 may be used as a biomarker for WG intake when the WG intake primarily comes from rye as in the present study. This trial was registered at clinicaltrials.gov as NCT01457794.
全谷物摄入对人体健康很重要，但准确估计摄入量具有挑战性。使用全谷物摄入生物标志物为验证膳食评估方法提供了一种可能的途径。
我们的目的是利用血浆烷基间苯二酚（AR）浓度验证儿童报告的两种饮食中的全谷物摄入量，并研究AR浓度和报告的全谷物摄入量的3个月重现性。
在一项2次学校膳食×3个月交叉试验中，对750名8至11岁参与者的空腹血浆样本中的AR浓度进行了分析，并通过基于网络的7天日记估计了全谷物摄入量。比较了儿童食用常规面包午餐（UBL）和热午餐（HLM）时报告的全谷物摄入量和血浆AR浓度。使用相关性和交叉分类根据摄入量对受试者进行排名。使用受试者在基线和UBL后测量值之间的组内相关系数（ICC）来评估重现性。
对于UBL和HLM饮食，报告的全谷物小麦+黑麦摄入量与血浆AR之间的相关性分别为0.40和0.37（P<0.001），对于UBL和HLM饮食，分别有78%和77%被归类在相同或相邻的四分位数中。血浆总ARs的3个月ICC为0.47（95%CI：0.38，0.55），报告的全谷物摄入量的ICC为0.64（95%CI：0.58，0.70）。当使用AR C17:0同系物作为生物标志物时，相关性更高，反映的是黑麦摄入量而非血浆总ARs[UBL：r = 0.47；HLM：r = 0.43，P<0.001；ICC = 0.51（95%CI：0.43，0.59）]。
儿童自我报告的全谷物小麦+黑麦摄入量与血浆AR浓度呈中等相关性。在全谷物摄入量和血浆AR浓度中发现了较大的个体内差异。当全谷物摄入量如本研究中主要来自黑麦时，AR同系物C17:0可作为全谷物摄入的生物标志物。该试验在clinicaltrials.gov上注册，注册号为NCT01457794。
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