Erusalimsky J D, Morris C, Perks K, Brown R, Brooks S, Rozengurt E
Imperial Cancer Research Fund, London, England.
FEBS Lett. 1989 Sep 11;255(1):149-53. doi: 10.1016/0014-5793(89)81079-8.
We have obtained sequence data from five proteolytic peptides totalling 102 amino acid residues of the 80 kDa protein kinase C substrate purified from rat brain. The amino acid sequences of these five peptides were compared with that deduced from a cDNA encoding the 87 kDa protein kinase C substrate from bovine brain. The overall amino acid sequence identity within the regions covered by our peptides is 54%. Two peptides aligned at the C- and N-termini of the bovine protein kinase C substrate with a very high degree of homology (more than 80% identity). Two other peptides exhibited 62% and 46% identity with two regions located in the C-terminal half of the bovine protein. The fifth peptide which contains the sequence PEQPEQPEQ did not reveal any similarity with the bovine protein. Based on the homologies of our experimentally determined sequences, which represent about 30% of the deduced sequence of the bovine protein, we suggest that although these protein kinase C substrates are not identical, they may belong to a family of related proteins.
我们已从从大鼠脑纯化的80 kDa蛋白激酶C底物的五条蛋白水解肽中获得了序列数据，这些肽总共包含102个氨基酸残基。将这五条肽的氨基酸序列与从编码牛脑87 kDa蛋白激酶C底物的cDNA推导的序列进行了比较。我们的肽所覆盖区域内的总体氨基酸序列同一性为54%。两条肽在牛蛋白激酶C底物的C端和N端对齐，具有非常高的同源性（同一性超过80%）。另外两条肽与牛蛋白C端一半区域的两个区域的同一性分别为62%和46%。包含序列PEQPEQPEQ的第五条肽与牛蛋白没有显示出任何相似性。基于我们实验确定的序列的同源性，这些序列约占牛蛋白推导序列的30%，我们认为，尽管这些蛋白激酶C底物并不相同，但它们可能属于相关蛋白家族。
