Ono Y, Kurokawa T, Kawahara K, Nishimura O, Marumoto R, Igarashi K, Sugino Y, Kikkawa U, Ogita K, Nishizuka Y
FEBS Lett. 1986 Jul 28;203(2):111-5. doi: 10.1016/0014-5793(86)80724-4.
Four peptides derived from rat brain protein kinase C were partially sequenced. Using synthetic oligonucleotides deduced from the amino acid sequences as probes, a clone of complementary DNA (cDNA) was isolated from a cDNA library prepared from the same tissue. The nucleotide sequence of this cDNA clone revealed the primary structure of the carboxyl-terminal region as having 224 amino acids, with significant sequence homology with cyclic AMP-dependent and cyclic GMP-dependent protein kinases.
从大鼠脑蛋白激酶C衍生出的四种肽段进行了部分测序。以从氨基酸序列推导的合成寡核苷酸作为探针，从同一组织制备的cDNA文库中分离出一个互补DNA（cDNA）克隆。该cDNA克隆的核苷酸序列揭示了羧基末端区域的一级结构有224个氨基酸，与环磷酸腺苷依赖性和环磷酸鸟苷依赖性蛋白激酶有显著的序列同源性。
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