Yamaguchi Takuya, Miyata Shunsuke, Katakura Fumihiko, Nagasawa Takahiro, Shibasaki Yasuhiro, Yabu Takeshi, Fischer Uwe, Nakayasu Chihaya, Nakanishi Teruyuki, Moritomo Tadaaki
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan; Laboratory of Fish Immunology, Institute of Infectology, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.
Laboratory of Fish Pathology, Department of Veterinary Medicine, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
Fish Shellfish Immunol. 2016 Feb;49:225-9. doi: 10.1016/j.fsi.2015.12.043. Epub 2016 Jan 4.
Teleost IL-4/13B is a cytokine related to mammalian IL-4 and IL-13, of which hitherto the function had not been studied at the protein level. We identified an IL-4/13B gene in common carp (Cyprinus carpio) and expressed the recombinant protein (rcIL-4/13B). RcIL-4/13B was shown to stimulate proliferation of IgM(+) B cells, because after four days of stimulation the IgM(+) fraction of carp kidney and spleen leukocytes had formed many cell colonies, whereas such colonies were not found in the absence of rcIL-4/13B stimulation. After nine days of incubation with rcIL-4/13B these cells had proliferated to more than 3-to-7-fold higher numbers when compared to untreated cells. The proliferating cells contained a majority of IgM(+) cells but also other cells, as indicated by FACS and RT-PCR analyses. The important conclusion is that in fish not only IL-4/13A has B cell stimulating properties, as a previous publication has shown, but also IL-4/13B.
硬骨鱼白细胞介素-4/13B是一种与哺乳动物白细胞介素-4和白细胞介素-13相关的细胞因子，迄今为止尚未在蛋白质水平上对其功能进行研究。我们在鲤鱼（Cyprinus carpio）中鉴定出白细胞介素-4/13B基因，并表达了重组蛋白（rcIL-4/13B）。结果表明，rcIL-4/13B能刺激IgM(+) B细胞增殖，因为在刺激四天后，鲤鱼肾脏和脾脏白细胞中的IgM(+)部分形成了许多细胞集落，而在没有rcIL-4/13B刺激的情况下未发现此类集落。与rcIL-4/13B孵育九天后，这些细胞的增殖数量比未处理的细胞高出3至7倍以上。通过荧光激活细胞分选术（FACS）和逆转录-聚合酶链反应（RT-PCR）分析表明，增殖细胞中大多数是IgM(+)细胞，但也包含其他细胞。重要的结论是，正如之前的一篇出版物所表明的，在鱼类中不仅白细胞介素-4/13A具有刺激B细胞的特性，白细胞介素-4/13B也具有该特性。
