Osaki T, Yokoe I, Ogura S, Takahashi K, Murakami K, Inoue K, Ishizuka M, Tanaka T, Li L, Sugiyama A, Azuma K, Murahata Y, Tsuka T, Ito N, Imagawa T, Okamoto Y
Joint Department of Veterinary Clinical Medicine, Faculty of Agriculture, Tottori University, Tottori, Japan.
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Vet Comp Oncol. 2017 Sep;15(3):731-739. doi: 10.1111/vco.12213. Epub 2016 Jan 14.
5-Aminolevulinic acid (5-ALA) is widely used in photodynamic detection (PDD) and therapy. We evaluated the pharmacokinetics of 5-ALA-induced porphyrins and its effectiveness in PDD in dogs with mammary gland tumours (MGTs) following oral administration. Healthy dogs and those with MGTs (nine each) were orally administered 4 mg kg 5-ALA. Protoporphyrin IX (PpIX) was not detected in the plasma of healthy dogs but it peaked in dogs with MGT at 2 h after 5-ALA administration. In the PDD study, 16 dogs with MGT were orally administered 40 mg kg 5-ALA, and MGT but not normal tissue showed red fluorescence after 2-4 h. Photon counts were 6635-63 890 and 59-4011 (median, 19 943 and 919) for MGT and non-tumour tissues, respectively. Cell density strongly correlated with PpIX photon counts of MGT tissue of the dogs (R = 0.743, P = 0.0009). We suggest that 5-ALA-PDD might be an effective diagnostic tool for MGTs.
5-氨基酮戊酸（5-ALA）广泛应用于光动力检测（PDD）和治疗。我们评估了口服给药后5-ALA诱导的卟啉在患有乳腺肿瘤（MGT）的犬体内的药代动力学及其在PDD中的有效性。健康犬和患有MGT的犬（各9只）口服4mg/kg的5-ALA。在健康犬的血浆中未检测到原卟啉IX（PpIX），但在患有MGT的犬中，5-ALA给药后2小时PpIX达到峰值。在PDD研究中，16只患有MGT的犬口服40mg/kg的5-ALA，2-4小时后，MGT而非正常组织呈现红色荧光。MGT和非肿瘤组织的光子计数分别为6635-63890和59-4011（中位数分别为19943和919）。细胞密度与犬MGT组织的PpIX光子计数密切相关（R = 0.743，P = 0.0009）。我们认为5-ALA-PDD可能是一种用于MGT的有效诊断工具。
