双带棕榈蝮（普通矛头蝮）毒液中的出血毒素。

Hemorrhagic toxin from the venom of Agkistrodon bilineatus (common cantil).

Imai K, Nikai T, Sugihara H, Ownby C L

Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.

Int J Biochem. 1989;21(6):667-73. doi: 10.1016/0020-711x(89)90388-1.

Abstract

Hemorrhagic toxin was isolated from Agkistrodon bilineatus (Common cantil) venom using a three-step purification procedure to obtain 32.8 mg of purified hemorrhagic toxin from 700 mg of crude venom. 2. The purified toxin was homogeneous by disc polyacrylamide gel electrophoresis at pH 8.3, and by isoelectric focusing. 3. Hemorrhagic toxin possessed lethal, hemorrhagic and proteolytic activities. These activities of this toxin were inhibited by ethylenediaminetetraacetic acid (EDTA) and ethyleneglycol-bis-(beta-aminoethylether)N,N'-tetraacetic acid (EGTA), but not by cysteine or soybean trypsin inhibitor (SBTI). 4. Its molecular weight was approximately 48 kDa and the isoelectric point was 4.2. 5. Purified preparation hydrolyzed the Asn(3)--Gln(4), His(10)--Leu(11), Ala(14)--Leu(15), Tyr(16)--Leu(17), Arg(22)--Gly(23) and Phe(24)--Phe(25) bonds of oxidized insulin B. chain. 6. The A alpha chain of fibrinogen was first split and B beta chain was cleaved later by this toxin. 7. Hemorrhagic toxin contains 1 mol of zinc and 2 mol of calcium per mol of protein.

摘要

采用三步纯化程序从双带腹链蛇（普通矛头蝮）毒液中分离出血毒素，从700毫克粗毒液中获得32.8毫克纯化的血毒素。2. 纯化后的毒素在pH 8.3的圆盘聚丙烯酰胺凝胶电泳和等电聚焦中均表现为均一性。3. 血毒素具有致死、出血和蛋白水解活性。该毒素的这些活性受到乙二胺四乙酸（EDTA）和乙二醇双（β-氨基乙基醚）N，N'-四乙酸（EGTA）的抑制，但不受半胱氨酸或大豆胰蛋白酶抑制剂（SBTI）的抑制。4. 其分子量约为48 kDa，等电点为4.2。5. 纯化制剂水解氧化胰岛素B链的Asn(3)--Gln(4)、His(10)--Leu(11)、Ala(14)--Leu(15)、Tyr(16)--Leu(17)、Arg(22)--Gly(23)和Phe(24)--Phe(25)键。6. 血毒素首先裂解纤维蛋白原的Aα链，随后裂解Bβ链。7. 每摩尔蛋白质，血毒素含有1摩尔锌和2摩尔钙。