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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Bando E, Nikai T, Sugihara H

Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.

Int J Biochem. 1991;23(11):1193-9. doi: 10.1016/0020-711x(91)90215-9.

A hemorrhagic protease I (HP-I) was isolated from Calloselasma rhodostoma venom by Sephadex G-75, DEAE-Sephacel and Q-Sepharose column chromatographies. 2. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis. 3. HP-I has a molecular weight of 34,800 and possesses hemorrhagic and proteolytic activities. Both activities are inhibited by ethylenediaminetetraacetic acid (EDTA), 1,10-phenanthroline, ethyleneglycolbis-(beta-aminoethyl ether) N,N'-tetraacetic acid (EGTA), and tetraethylenepentamine (TEP). However neither soybean trypsin inhibitor nor p-chlorobenzoic acid (PCMB) were found to have any effect. 4. The toxin contains 311 amino acid residues and exhibits an isoelectric point of 4.5. 5. The A alpha chain of fibrinogen was cleaved first, followed later by the B beta chain.

通过葡聚糖凝胶G - 75、二乙氨基乙基葡聚糖凝胶（DEAE - Sephacel）和Q - 琼脂糖柱色谱法从圆斑蝰蛇毒中分离出一种出血性蛋白酶I（HP - I）。2. 通过在丙烯酰胺凝胶电泳中形成单一谱带来确定其均一性。3. HP - I的分子量为34,800，具有出血活性和蛋白水解活性。这两种活性均受到乙二胺四乙酸（EDTA）、1,10 - 菲咯啉、乙二醇双（β - 氨基乙醚）N,N' - 四乙酸（EGTA）和四乙烯五胺（TEP）的抑制。然而，未发现大豆胰蛋白酶抑制剂和对氯苯甲酸（PCMB）有任何作用。4. 该毒素含有311个氨基酸残基，等电点为4.5。5. 纤维蛋白原的Aα链首先被切割，随后是Bβ链。

Bando E, Nikai T, Sugihara H

Department of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.

Int J Biochem. 1991;23(11):1193-9. doi: 10.1016/0020-711x(91)90215-9.

A hemorrhagic protease I (HP-I) was isolated from Calloselasma rhodostoma venom by Sephadex G-75, DEAE-Sephacel and Q-Sepharose column chromatographies. 2. Homogeneity was established by the formation of a single band in acrylamide gel electrophoresis. 3. HP-I has a molecular weight of 34,800 and possesses hemorrhagic and proteolytic activities. Both activities are inhibited by ethylenediaminetetraacetic acid (EDTA), 1,10-phenanthroline, ethyleneglycolbis-(beta-aminoethyl ether) N,N'-tetraacetic acid (EGTA), and tetraethylenepentamine (TEP). However neither soybean trypsin inhibitor nor p-chlorobenzoic acid (PCMB) were found to have any effect. 4. The toxin contains 311 amino acid residues and exhibits an isoelectric point of 4.5. 5. The A alpha chain of fibrinogen was cleaved first, followed later by the B beta chain.

通过葡聚糖凝胶G - 75、二乙氨基乙基葡聚糖凝胶（DEAE - Sephacel）和Q - 琼脂糖柱色谱法从圆斑蝰蛇毒中分离出一种出血性蛋白酶I（HP - I）。2. 通过在丙烯酰胺凝胶电泳中形成单一谱带来确定其均一性。3. HP - I的分子量为34,800，具有出血活性和蛋白水解活性。这两种活性均受到乙二胺四乙酸（EDTA）、1,10 - 菲咯啉、乙二醇双（β - 氨基乙醚）N,N' - 四乙酸（EGTA）和四乙烯五胺（TEP）的抑制。然而，未发现大豆胰蛋白酶抑制剂和对氯苯甲酸（PCMB）有任何作用。4. 该毒素含有311个氨基酸残基，等电点为4.5。5. 纤维蛋白原的Aα链首先被切割，随后是Bβ链。