对从大肠杆菌UKT - B脂多糖中分离出的R型和S型脂多糖进行热原和鲎试剂检测的比较。

Comparison of R- and S-form lipopolysaccharides fractionated from Escherichia coli UKT-B lipopolysaccharide in pyrogen and Limulus tests.

作者信息

Komuro T, Yomota C, Kimura T, Galanos C

机构信息

National Institute of Hygienic Sciences, Osaka Branch, Japan.

出版信息

FEMS Microbiol Lett. 1989 Jul 1;51(1):79-83. doi: 10.1016/0378-1097(89)90081-5.

DOI:10.1016/0378-1097(89)90081-5

PMID:2676711

Abstract

Different LPS was shown to have a relatively different proportion of O-specific chain-less (R-form) LPS by polyacrylamide gel electrophoresis (PAGE) with sodium deoxycholate (DOC). By using DOC-PAGE, S-form LPS having O-chain with approximately 11 repeating units on average (S-Fr) and O-chain-less LPS (R-Fr) were separated from Escherichia coli UKT-B S-form LPS. Significantly stronger pyrogenicity was observed in R-Fr than in S-Fr when measured on the weight basis. Similar result was observed in Limulus test. Comparing biological activities of different S-form LPS, attention should be given to the amounts of co-existing R-form LPS.

摘要

通过使用脱氧胆酸钠（DOC）进行聚丙烯酰胺凝胶电泳（PAGE）显示，不同的脂多糖（LPS）具有相对不同比例的无O特异性链（R型）LPS。通过DOC-PAGE，从大肠杆菌UKT-B S型LPS中分离出平均具有约11个重复单元O链的S型LPS（S-Fr）和无O链LPS（R-Fr）。以重量为基础测量时，观察到R-Fr的致热活性明显强于S-Fr。在鲎试剂检测中也观察到类似结果。比较不同S型LPS的生物活性时，应注意共存R型LPS的量。