来自白色链霉菌的编码嘌呤霉素N-乙酰基转移酶的pac基因的分子分析。

Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger.

作者信息

Lacalle R A, Pulido D, Vara J, Zalacaín M, Jiménez A

机构信息

Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Canto Blanco, Madrid, Spain.

出版信息

Gene. 1989 Jul 15;79(2):375-80. doi: 10.1016/0378-1119(89)90220-5.

DOI:10.1016/0378-1119(89)90220-5

PMID:2676728

Abstract

Nucleotide sequence of a 906-bp fragment of Streptomyces alboniger DNA containing the gene (pac), which encodes a puromycin N-acetyltransferase (PAC), has been determined. The pac gene contains a 600-nt open reading frame, starting with an ATG codon, which encodes a polypeptide of Mr 21,531; this is consistent with the 23 +/- 1.5 kDa size of the PAC enzyme. High-resolution S1 mapping indicates that transcription starts at or next to a C residue 35 bp upstream from the putative ATG start codon. A 263-bp DNA fragment from the 5' region of the pac gene has promoter activity in the promoter-probe plasmid pIJ486. Its -35 and -10 regions show significant structural homology to the corresponding regions of the hyg gene promoter, but they are different from the promoter sequences of other Streptomyces and Escherichia coli genes.

摘要

已测定了包含编码嘌呤霉素N - 乙酰转移酶（PAC）的基因（pac）的白色链霉菌DNA 906 bp片段的核苷酸序列。pac基因包含一个600 nt的开放阅读框，起始于一个ATG密码子，编码一个Mr为21,531的多肽；这与PAC酶23±1.5 kDa的大小一致。高分辨率S1图谱表明转录起始于假定的ATG起始密码子上游35 bp处的一个C残基或其紧邻位置。来自pac基因5'区域的一个263 bp DNA片段在启动子探针质粒pIJ486中具有启动子活性。其-35和-10区域与潮霉素基因启动子的相应区域显示出显著的结构同源性，但与其他链霉菌和大肠杆菌基因的启动子序列不同。