来自吸水链霉菌的潮霉素基因启动子：链霉菌启动子的一种新形式。

The hyg gene promoter from Streptomyces hygroscopicus: a novel form of Streptomyces promoters.

作者信息

Pulido D, Zalacaín M, Jiménez A

机构信息

Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Canto Blanco, Madrid, Spain.

出版信息

Biochem Biophys Res Commun. 1988 Feb 29;151(1):270-4. doi: 10.1016/0006-291x(88)90589-x.

DOI:10.1016/0006-291x(88)90589-x

PMID:2831883

Abstract

A 207 bp DNA fragment from the 5' region of the hyg gene of Streptomyces hygroscopicus was located preceding a DNA sequence encoding the mature form of human interferon alpha 2. This gene fusion, inserted in the Streptomyces vector pIJ702, expressed interferon activity in Streptomyces lividans indicating that the 207 bp sequence has promoter activity. The transcription initiation site was located. No significant homology with previously described Streptomyces promoters could be found. It appears therefore, it represents a novel class of Streptomyces promoters.

摘要

来自吸水链霉菌潮霉素基因5'区域的一段207 bp的DNA片段位于编码人α2干扰素成熟形式的DNA序列之前。这个基因融合体插入链霉菌载体pIJ702后，在变铅青链霉菌中表达干扰素活性，表明该207 bp序列具有启动子活性。转录起始位点已定位。未发现与先前描述的链霉菌启动子有明显同源性。因此，它似乎代表了一类新型的链霉菌启动子。