[盐知母的高效液相色谱-光电二极管阵列-蒸发光散射检测器指纹图谱]
[HPLC-PDA-CAD Fingerprints of Salt Anemarrhenae Rhizoma].
作者信息
Wu Ying, Gao Hui, Song Ze-bi
出版信息
Zhong Yao Cai. 2015 May;38(5):942-7.
OBJECTIVE
To establish the HPLC-PDA-CAD fingerprints and to determine its seven main constituents so as to provide a reliable evidence for the scientific evaluation and quality control of salt Anemarrhenae Rhizoma.
METHODS
The chromatographic fingerprint was obtained with Thermo Hypersil C18 column (250 mm x 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water; The CAD parameters were pressure of 241. 3 kPa, filter of high and range of 200 pA. The detection wavelength of PDA was set at 258 nm.
RESULTS
The common mode of HPLC-PDA-CAD fingerprint of salt Anemarrhenae Rhizoma was set up. There were 5 PDA and 12 CAD common peaks in the fingerprints. Timosaponin B II, anemarsaponin E, timosaponin B III, timosaponin A III, neomangiferin, mangiferin and baohuoside I were identified in fingerprints and determined.
CONCLUSION
The established HPLC-PDA-CAD fingerprint method is accurate, reliable, and has a good reproducibility and precision, which can be used for the quality control of salt Anemarrhenae Rhizoma.
目的
建立盐知母高效液相色谱-光电二极管阵列检测器-蒸发光散射检测器（HPLC-PDA-CAD）指纹图谱，并测定其7种主要成分，为盐知母的科学评价和质量控制提供可靠依据。
方法
采用Thermo Hypersil C18色谱柱（250 mm×4.6 mm，5 μm），以乙腈和水为流动相进行梯度洗脱；蒸发光散射检测器（CAD）参数为压力241.3 kPa、过滤器高、范围200 pA。光电二极管阵列检测器（PDA）检测波长设定为258 nm。
结果
建立了盐知母HPLC-PDA-CAD指纹图谱的共有模式。指纹图谱中有5个PDA共有峰和12个CAD共有峰。鉴定并测定了指纹图谱中的知母皂苷BⅡ、知母皂苷E、知母皂苷BⅢ、知母皂苷AⅢ、新芒果苷、芒果苷和宝藿苷Ⅰ。
结论
所建立的HPLC-PDA-CAD指纹图谱方法准确、可靠，具有良好的重复性和精密度，可用于盐知母的质量控制。
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