Wei Qiang, Ji Xiao-ying, Xu Fei, Li Qian-rong, Yin Hao
Zhong Yao Cai. 2015 May;38(5):975-9.
To study the chemical constituents from Hibiscus syriacus leaves and their α-glucosidase inhibitory activities.
Column chromatography including macroporous resins, silica gel and Sephadex LH-20 were used for the isolation and purification of all compounds. Spectroscopic methods including physical and chemical properties, 1H-NMR and 13C-NMR were used for the identification of structures. Their α-glucosidase inhibitory activities were detected by a 96-well microplate.
15 compounds were isolated and identified as β-sitosterol(1), β-daucostero (2), β-amyrin (3), oleanolic acid (4), stigmast-4-en-3-one (5), friedelin (6), syriacusin A (7), kaempferol (8), isovitexin (9), vitexin (10), apigenin (11), apigenin-7-O-β-D-glucopyranoside (12), luteolin-7-O-β-D-glucopyranoside (13), vitexin-7-O-β-D-glucopyranoside (14) and rutin (15).
All the compounds are isolated from the leaves of Hibiscus syriacus for the first time. Taking acarbose as positive control, the α-glucosidase inhibitory activities of 15 compounds were evaluated. Compounds 7 and 9 have shown strong α-glucosidase inhibitory activities with IC50 of 39.03 ± 0.38 and 32.12 ± 0.62 mg/L, inhibition ratio of 94.95% and 97.15%, respectively.
研究木槿叶的化学成分及其α-葡萄糖苷酶抑制活性。
采用大孔树脂、硅胶和葡聚糖凝胶LH-20柱色谱法对所有化合物进行分离纯化。利用包括物理化学性质、1H-NMR和13C-NMR在内的光谱方法鉴定结构。通过96孔微孔板检测它们的α-葡萄糖苷酶抑制活性。
分离鉴定出15种化合物，分别为β-谷甾醇（1）、β-胡萝卜苷（2）、β-香树脂醇（3）、齐墩果酸（4）、豆甾-4-烯-3-酮（5）、木栓酮（6）、木槿素A（7）、山柰酚（8）、异荭草素（9）、荭草素（10）、芹菜素（11）、芹菜素-7-O-β-D-葡萄糖苷（12）、木犀草素-7-O-β-D-葡萄糖苷（13）、荭草素-7-O-β-D-葡萄糖苷（14）和芦丁（15）。
所有化合物均首次从木槿叶中分离得到。以阿卡波糖为阳性对照，评价了15种化合物的α-葡萄糖苷酶抑制活性。化合物7和9表现出较强的α-葡萄糖苷酶抑制活性，IC50分别为39.03±0.38和32.12±0.62mg/L，抑制率分别为94.95%和97.15%。
