Jiang Liuming, Wu Qun, Yang Tao
Department of Anesthesiology, The First Affiliated Hospital of Wenzhou Medical University, 2 Fuxue Road, Wenzhou, Zhejiang, 325000, China.
J Mol Neurosci. 2016 May;59(1):99-105. doi: 10.1007/s12031-016-0713-z. Epub 2016 Jan 15.
Inhibitor of DNA binding/differentiation 2 (Id2) belongs to a helix-loop-helix family of proteins. Recent studies have showed that Id2 plays a pivotal role in neuronal survival and neuroprotection. However, under neuropathic pain conditions, the role of Id2 is still unclear. In this study, we investigated the effect of Id2 on neuropathic pain in a rat chronic constriction injury (CCI) model. Our results demonstrated that Id2 was upregulated in the dorsal root ganglion (DRG) in a CCI rat in a time-dependent manner. Intrathecal short-hairpin RNA (shRNA)-Id2 attenuates mechanical allodynia and thermal hyperalgesia in CCI rats, and inhibits the expression of TNF-α and IL-1β in the DRG in CCI rats. Furthermore, knockdown of Id2 reduces the expression of NF-κB p65 in the DRG of CCI rats. Taken together, our findings suggest that knockdown of Id2 may alleviate neuropathic pain by inhibiting the NF-κB activation to inhibit the production of pro-inflammatory mediators. Therefore, Id2 may provide an important target of neuropathic pain treatment.
DNA结合/分化抑制因子2（Id2）属于螺旋-环-螺旋蛋白家族。最近的研究表明，Id2在神经元存活和神经保护中起关键作用。然而，在神经性疼痛条件下，Id2的作用仍不清楚。在本研究中，我们在大鼠慢性压迫损伤（CCI）模型中研究了Id2对神经性疼痛的影响。我们的结果表明，Id2在CCI大鼠的背根神经节（DRG）中呈时间依赖性上调。鞘内注射短发夹RNA（shRNA）-Id2可减轻CCI大鼠的机械性异常性疼痛和热痛觉过敏，并抑制CCI大鼠DRG中TNF-α和IL-1β的表达。此外，敲低Id2可降低CCI大鼠DRG中NF-κB p65的表达。综上所述，我们的研究结果表明敲低Id2可能通过抑制NF-κB激活来抑制促炎介质的产生，从而减轻神经性疼痛。因此，Id2可能为神经性疼痛治疗提供一个重要靶点。
