Kim J Y, Kye S J, Lee H J, Gaikwad S, Lee H S, Jung S C, Choi K S
Avian Disease Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Foot and Mouth Disease Diagnosis Division, Animal and Plant Quarantine Agency, Anyang, Republic of Korea.
Poult Sci. 2016 Apr;95(4):790-7. doi: 10.3382/ps/pev377. Epub 2016 Jan 14.
Newcastle disease virus (NDV) strain NDRL0901 was developed as a live vaccine candidate for control of Newcastle disease. NDV isolate KR/duck/13/07 (DK1307) of duck origin was used as the selected vaccine strain. DK1307 was passaged 6 times in chickens. Then a single clone from the chicken-adapted virus (DK1307C) was finally selected, and the vaccine strain was named NDRL0901. DK1307C and the clone NDRL0901 viruses showed enhanced immunogenicity compared to the DK1307 virus. Principal component analysis based on fusion and hemagglutinin-neuraminidase genes revealed the codon usage pattern in the dataset is distinct separating duck viral sequences and avian sequences, and passage of the duck origin virus into the chicken host causes deviation in the codon usage pattern. The NDRL0901 virus was avirulent and did not acquire viral virulence even after 7 back passages in chickens. When day-old chicks were vaccinated with the NDRL0901 virus via spray, eye drops, and drinking water, the vaccinated birds showed no clinical signs and had significant protection efficacy (>80%) against very virulent NDV (Kr005 strain) infection regardless of the administration route employed. The results indicate that the NDRL0901 strain is safe in chickens and can offer protective immunity.
新城疫病毒（NDV）毒株NDRL0901被开发为一种用于控制新城疫的活疫苗候选株。选用鸭源的NDV分离株KR/duck/13/07（DK1307）作为疫苗株。DK1307在鸡体内传代6次。然后从鸡适应病毒（DK1307C）中最终筛选出一个单克隆，该疫苗株被命名为NDRL0901。与DK1307病毒相比，DK1307C和克隆NDRL0901病毒表现出增强的免疫原性。基于融合基因和血凝素 - 神经氨酸酶基因的主成分分析表明，数据集中的密码子使用模式明显区分鸭病毒序列和禽病毒序列，鸭源病毒在鸡宿主中的传代导致密码子使用模式出现偏差。NDRL0901病毒无毒力，即使在鸡体内连续7次回传后也未获得病毒毒力。当1日龄雏鸡通过喷雾、滴眼和饮水方式接种NDRL0901病毒时，接种的鸡未表现出临床症状，并且无论采用何种给药途径，对强毒NDV（Kr005株）感染均具有显著的保护效力（>80%）。结果表明，NDRL0901株在鸡体内是安全的，并且可以提供保护性免疫。
