Gao Caixia, Han Jin, Liu Zhenmin, Xu Xiaofen, Hang Feng, Wu Zhengjun
State Key Laboratory of Dairy Biotechnology,Shanghai 200436,PR China.
Shanghai Engineering Research Center of Dairy Biotechnology,Shanghai 200436,PR China.
Int J Syst Evol Microbiol. 2016 Mar;66(3):1413-1418. doi: 10.1099/ijsem.0.000900. Epub 2016 Jan 13.
Strain BD3526, isolated from raw yak milk collected in Tibet, China, was studied to determine its taxonomic status. The strain was Gram-reaction positive, motile, catalase-positive, aerobic or facultatively anaerobic. The DNA G+C content of the strain was 47.5 mol% and its peptidoglycan contained -diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C, anteiso-C and C. Based on 16S rRNA gene sequence analysis, similarities among strain BD3526 and its most closely related species, A9, '' H9, '' Y24 and DSM 22170 were 99.0, 98.5, 97.3 and 96.7 %, respectively. Levels of DNA-DNA relatedness among strain BD3526 and DSM 22170 and ATCC 842 ( = DSM 36), the type species of the genus, were 41.2 and 45.6 %, respectively. genome-to-genome comparison showed that the DNA-DNA hybridization values among strain BD3526 and A9, '' H9 and '' Y24 were lower than 70 %. Based on its molecular and physiological properties, strain BD3526 ( = DSM 28815 = CGMCC 8333) is identified as the type strain of a novel species within the genus , for which the name is proposed.
对从中国西藏采集的牦牛奶中分离出的BD3526菌株进行了研究，以确定其分类地位。该菌株革兰氏反应呈阳性，具运动性，过氧化氢酶呈阳性，为需氧或兼性厌氧。该菌株的DNA G+C含量为47.5mol%，其肽聚糖含有二氨基庚二酸。主要的甲基萘醌是MK-7。主要的细胞脂肪酸是anteiso-C、anteiso-C和C。基于16S rRNA基因序列分析，BD3526菌株与其最密切相关的物种A9、H9、Y24和DSM 22170之间的相似性分别为99.0%、98.5%、97.3%和96.7%。BD3526菌株与DSM 22170以及该属模式种ATCC 842（=DSM 36）之间的DNA-DNA相关性水平分别为41.2%和45.6%。全基因组比较表明，BD3526菌株与A9、H9和Y24之间的DNA-DNA杂交值低于70%。基于其分子和生理特性，BD3526菌株（=DSM 28815=CGMCC 8333）被鉴定为该属内一个新物种的模式菌株，为此提出了该名称。
