Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading RG6 6AP, UK.
Department of Plant Science, Rothamsted Research, Harpenden AL5 2JQ, Hertfordshire, UK.
Food Chem. 2016 May 1;198:12-9. doi: 10.1016/j.foodchem.2015.11.036. Epub 2015 Nov 11.
Wheat Distillers' Dried Grains with Solubles (DDGS) and in-process samples were used for protein extraction. Prolamins were the predominant protein components in the samples. The absence of extractable α- and γ-gliadins in DDGS indicated protein aggregation during the drum drying processing stage. Prolamin extraction was performed using 70% (v/v) ethanol or alkaline-ethanol solution in the presence of reducing agent. DDGS extracts had relatively low protein contents (14-44.9%, w/w), regardless of the condition applied. The wet solids were the most suitable raw material for protein extraction, with recovery yields of ∼ 55% (w/w) and protein content of ∼ 58% (w/w) in 70% (v/v) ethanol. Protein extracts from wet solids were significantly rich in glutamic acid and proline. Mass balance calculations demonstrated the high carbohydrate content (∼ 50%, w/w) of solid residues. Overall, the feasibility of utilising in-process samples of DDGS for protein extraction with commercial potential was demonstrated.
利用小麦干酒糟及其可溶物（DDGS）和中间样品进行蛋白质提取。醇溶蛋白是样品中主要的蛋白质成分。在鼓式干燥加工阶段，DDGS 中可提取的α-和γ-麦谷蛋白缺失表明发生了蛋白质聚集。在还原剂存在的情况下，使用 70%（v/v）乙醇或碱性乙醇溶液进行醇溶蛋白提取。无论应用条件如何，DDGS 提取物的蛋白质含量都相对较低（14-44.9%，w/w）。湿固体是最适合蛋白质提取的原料，在 70%（v/v）乙醇中，提取收率约为 55%（w/w），蛋白质含量约为 58%（w/w）。来自湿固体的蛋白质提取物富含谷氨酸和脯氨酸。物料平衡计算表明固体残留物的碳水化合物含量较高（约 50%，w/w）。总体而言，证明了利用 DDGS 中间样品进行具有商业潜力的蛋白质提取的可行性。
