奶酪乳清：兽疫链球菌生产透明质酸的一种具有成本效益的替代物。
Cheese whey: A cost-effective alternative for hyaluronic acid production by Streptococcus zooepidemicus.
机构信息
Departamento de Química Analítica y Alimentaria, Universidad de Vigo, Ourense, Spain; CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.
Grupo de Reciclado y Valorización de Materiales Residuales, IIM-CSIC, Vigo, Spain.
出版信息
Food Chem. 2016 May 1;198:54-61. doi: 10.1016/j.foodchem.2015.11.062. Epub 2015 Nov 18.
This study focuses on the optimisation of cheese whey formulated media for the production of hyaluronic acid (HA) by Streptococcus zooepidemicus. Culture media containing whey (W; 2.1g/L) or whey hydrolysate (WH; 2.4 g/L) gave the highest HA productions. Both W and WH produced high yields on protein consumed, suggesting cheese whey is a good nitrogen source for S. zooepidemicus production of HA. Polysaccharide concentrations of 4.0 g/L and 3.2g/L were produced in W and WH in a further scale-up to 5L bioreactors, confirming the suitability of the low-cost nitrogen source. Cheese whey culture media provided high molecular weight (>3000 kDa) HA products. This study revealed replacing the commercial peptone by the low-cost alternative could reduce HA production costs by up to a 70% compared to synthetic media.
本研究致力于优化奶酪乳清配方培养基，以生产由兽疫链球菌（Streptococcus zooepidemicus）产生的透明质酸（HA）。含有乳清（W；2.1g/L）或乳清水解物（WH；2.4g/L）的培养基可实现最高的 HA 产量。W 和 WH 均在消耗的蛋白质上产生了高产量，表明奶酪乳清是兽疫链球菌生产 HA 的良好氮源。在进一步扩大到 5L 生物反应器中，W 和 WH 中分别产生了 4.0g/L 和 3.2g/L 的多糖浓度，证实了这种低成本氮源的适用性。奶酪乳清培养基提供了高分子量（>3000 kDa）的 HA 产品。本研究表明，用低成本替代物替代商业蛋白胨可以将 HA 的生产成本降低 70%，与合成培养基相比。
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