Lee Sang In, Kim Hyun Soo, Koo Jin Mo, Kim In Ho
Department of Animal Resource and Science,Dankook University,Cheonan,Choongnam,330-714,Republic of Korea.
Br J Nutr. 2016 Feb 28;115(4):567-75. doi: 10.1017/S0007114515004857. Epub 2016 Jan 15.
A total of forty weaned pigs ((Landrace × Yorkshire) × Duroc) were used to evaluate the effects of Lactobacillus acidophilus on inflammatory activity after lipopolysaccharide (LPS) challenge. Experimental treatments were as follows: (T1) control diet+saline challenge; (T2) control diet with 0·1% L. acidophilus+saline challenge; (T3) control diet+LPS challenge; and (T4) control diet with 0·1% L. acidophilus+LPS challenge. On d-14, piglets were challenged with saline (T1 and T2) or LPS (T3 and T4). Blood samples were obtained at 0, 2, 4, 6 and 12 h after being challenged and analysed for immune cell cytokine production and gene expression pattern. The L. acidophilus treatment increased the average daily weight gain (ADWG) and average daily feed intake (ADFI) compared with the control diet. With the control diet, the LPS challenge (T3) increased the number of immune cells and expression of TNF-α and IL-6 compared with the saline challenge (T1). Whereas with the saline challenge L. acidophilus treatment (T2) increased the number of leucocytes and CD4 compared with the control diet (T1), with the LPS challenge L. acidophilus treatment (T4) decreased the number of leucocytes, lymphocytes, CD4+ and CD8+ and expression of TNF-α and IL-6 compared with the control diet (T3). L. acidophilus treatment decreased the expression of TRL4 and NF-κB in peripheral blood mononuclear cells (PBMC) after LPS challenge, which leads to inhibition of TNF-α, IFN-γ, IL-6, IL-8 and IL1B1 and to induction of IL-4 and IL-10. We suggested that L. acidophilus improved ADWG and ADFI and protected against LPS-induced inflammatory responses by regulating TLR4 and NF-κB expression in porcine PBMC.
总共40头断奶仔猪（（长白猪×大白猪）×杜洛克猪）用于评估嗜酸乳杆菌对脂多糖（LPS）攻击后炎症活性的影响。实验处理如下：（T1）对照日粮+生理盐水攻击；（T2）含0.1%嗜酸乳杆菌的对照日粮+生理盐水攻击；（T3）对照日粮+LPS攻击；以及（T4）含0.1%嗜酸乳杆菌的对照日粮+LPS攻击。在第14天，仔猪接受生理盐水（T1和T2）或LPS（T3和T4）攻击。在攻击后0、2、4、6和12小时采集血样，分析免疫细胞细胞因子产生和基因表达模式。与对照日粮相比，嗜酸乳杆菌处理提高了平均日增重（ADWG）和平均日采食量（ADFI）。采用对照日粮时，与生理盐水攻击（T1）相比，LPS攻击（T3）增加了免疫细胞数量以及TNF-α和IL-6的表达。而在生理盐水攻击时，与对照日粮（T1）相比，嗜酸乳杆菌处理（T2）增加了白细胞数量和CD4数量；在LPS攻击时，与对照日粮（T3）相比，嗜酸乳杆菌处理（T4）减少了白细胞、淋巴细胞、CD4+和CD8+数量以及TNF-α和IL-6的表达。LPS攻击后，嗜酸乳杆菌处理降低了外周血单核细胞（PBMC）中TRL4和NF-κB的表达，这导致TNF-α、IFN-γ、IL-6、IL-8和IL1B1受到抑制，并诱导IL-4和IL-10产生。我们认为，嗜酸乳杆菌通过调节猪PBMC中TLR4和NF-κB的表达，改善了ADWG和ADFI，并预防了LPS诱导的炎症反应。
