Chowra Umakanta, Yanase Emiko, Koyama Hiroyuki, Panda Sanjib Kumar
Plant Molecular Biotechnology Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, 788011, India.
United Graduate School of Agricultural Science, Faculty of Applied Biological Science, Gifu University, Gifu, Japan.
Protoplasma. 2017 Jan;254(1):293-302. doi: 10.1007/s00709-016-0943-5. Epub 2016 Jan 15.
Aluminium-induced oxidative damage caused by excessive ROS production was evaluated in black gram pulse crop. Black gram plants were treated with different aluminium (Al) concentrations (10, 50 and 100 μM with pH 4.7) and further the effects of Al were characterised by means of root growth inhibition, histochemical assay, ROS content analysis, protein carbonylation quantification and H-NMR analysis. The results showed that aluminium induces excessive ROS production which leads to cellular damage, root injury, stunt root growth and other metabolic shifts. In black gram, Al induces cellular damage at the earliest stage of stress which was characterised from histochemical analysis. From this study, it was observed that prolonged stress can activate certain aluminium detoxification defence mechanism. Probably excessive ROS triggers such defence mechanism in black gram. Al can induce excessive ROS initially in the root region then transported to other parts of the plant. As much as the Al concentration increases, the rate of cellular injury and ROS production also increases. But after 72 h of stress, plants showed a lowered ROS level and cellular damage which indicates the upregulation of defensive mechanisms. Metabolic shift analysis also showed that the black gram plant under stress has less metabolic content after 24 h of treatment, but gradually, it was increased after 72 h of treatment. It was assumed that ROS played the most important role as a signalling molecule for aluminium stress in black gram.
在黑绿豆作物中评估了由过量活性氧（ROS）产生引起的铝诱导的氧化损伤。用不同铝（Al）浓度（10、50和100μM，pH 4.7）处理黑绿豆植株，进一步通过根生长抑制、组织化学分析、ROS含量分析、蛋白质羰基化定量和氢核磁共振（H-NMR）分析来表征铝的影响。结果表明，铝诱导过量ROS产生，导致细胞损伤、根系损伤、根系生长受阻和其他代谢变化。在黑绿豆中，铝在胁迫的最早阶段就诱导细胞损伤，这从组织化学分析中得以表征。从这项研究中观察到，长期胁迫可激活某些铝解毒防御机制。可能过量的ROS在黑绿豆中触发了这种防御机制。铝最初可在根部区域诱导过量ROS产生，然后运输到植物的其他部位。随着铝浓度的增加，细胞损伤率和ROS产生率也增加。但在胁迫72小时后，植株的ROS水平和细胞损伤降低，这表明防御机制上调。代谢变化分析还表明，胁迫下的黑绿豆植株在处理24小时后代谢物含量较少，但在处理72小时后逐渐增加。据推测，ROS在黑绿豆中作为铝胁迫的信号分子发挥了最重要的作用。
