Department of Environmental Engineering, University of Seoul, Seoul, Republic of Korea.
Department of Environmental Engineering, University of Seoul, Seoul, Republic of Korea.
Ecotoxicol Environ Saf. 2018 Dec 15;165:423-433. doi: 10.1016/j.ecoenv.2018.09.033. Epub 2018 Sep 13.
Wide use of AlO nanoparticles (NPs) leading to their possible escape into environment and their interaction with living organisms demands immediate attention. We evaluated impact of nanoparticulate (AlO-NPs) and ionic (Al) forms of aluminium on early seedling growth of Vigna radiata. While Al inhibited growth of seedlings, AlO-NPs did not affect it negatively. Unlike enhancement in proline, malondialdehyde and HO levels in roots and shoots induced by Al, these stress markers remained unaltered by AlO-NPs. No signs of membrane damage were recorded in roots of seedlings raised in presence of AlO-NPs; this was witnessed from insignificant electrolyte leakage and Evans blue uptake. Activities of antioxidant enzymes, i.e., superoxide dismustase, catalase, guaiacol peroxidase in root and shoot were enhanced by Al. However, they were unaffected by AlO-NPs. Al enhanced levels of non-protein thiols, phenolics and ascorbate, with no alterations induced by AlO-NPs. These findings revealed that, AlO-NPs did not induce oxidative stress in seedlings. Seedlings raised in Al showed higher uptake of Al than those grown in AlO-NPs; Al content was higher in roots. Al was not detected in shoots of seedlings grown in AlO-NPs. Lower translocation of Al in seedlings raised in AlO-NPs was due to adsorption/restriction of AlO-NPs on root surface. Al caused ruptures on root epidermis of seedlings and inhibited root-hair formation, whereas no structural damage was caused by AlO-NPs. Our findings revealed that while ionic Al is highly toxic, nanoparticulate form of Al is non-toxic to growth of V. radiata.
广泛使用 AlO 纳米颗粒（NPs）导致它们可能逃逸到环境中，并与生物体相互作用，这需要立即引起关注。我们评估了纳米颗粒（AlO-NPs）和离子（Al）形式的铝对豇豆早期幼苗生长的影响。虽然铝抑制了幼苗的生长，但 AlO-NPs 并没有对其产生负面影响。与铝诱导的根和芽中脯氨酸、丙二醛和 HO 水平的增强不同，这些应激标志物在 AlO-NPs 作用下没有发生变化。在 AlO-NPs 存在下培养的幼苗根部没有记录到膜损伤的迹象；这从无意义的电解质泄漏和 Evans 蓝摄取中可以看出。抗氧化酶，即超氧化物歧化酶、过氧化氢酶、根和芽中的愈创木酚过氧化物酶的活性被铝增强。然而，它们不受 AlO-NPs 的影响。铝增强了非蛋白巯基、酚类和抗坏血酸的水平，而 AlO-NPs 没有引起变化。这些发现表明，AlO-NPs 不会在幼苗中诱导氧化应激。在 Al 中生长的幼苗比在 AlO-NPs 中生长的幼苗吸收更多的 Al；Al 在根部的含量更高。在 AlO-NPs 中生长的幼苗的茎中没有检测到 Al。在 AlO-NPs 中生长的幼苗中 Al 的较低迁移率是由于 AlO-NPs 在根表面的吸附/限制。Al 导致幼苗根表皮破裂并抑制根毛形成，而 AlO-NPs 没有造成结构损伤。我们的研究结果表明，虽然离子 Al 具有高度毒性，但纳米形式的 Al 对豇豆的生长无毒。
