Kyriakides Michael, Rama Nona, Sidhu Jasmin, Gabra Hani, Keun Hector C, El-Bahrawy Mona
Department of Surgery and Cancer, Imperial College London, London, United Kingdom.
Department of Histopathology, Hammersmith Hospital, Imperial College London, London, United Kingdom.
Oncotarget. 2016 Feb 9;7(6):7216-26. doi: 10.18632/oncotarget.6891.
The majority of ovarian tumours are of the epithelial type, which can be sub classified as benign, borderline or malignant. Epithelial tumours usually have cystic spaces filled with cyst fluid, the metabolic profile of which reflects the metabolic activity of the tumour cells, due to their close proximity. The approach of metabonomics using 1H-NMR spectroscopy was employed to characterize the metabolic profiles of ovarian cyst fluid samples (n = 23) from benign, borderline and malignant ovarian tumours in order to shed more light into ovarian tumour and cancer development. The analysis revealed that citrate was elevated in benign versus malignant tumours, while the amino acid lysine was elevated in malignant versus non-malignant tumours, both at a 5% significance level. Choline and lactate also had progressively increasing levels from benign to borderline to malignant samples. Finally, hypoxanthine was detected exclusively in a sub-cohort of the malignant tumours. This metabonomic study demonstrates that ovarian cyst fluid samples have potential to be used to distinguish between the different types of ovarian epithelial tumours. Furthermore, the respective metabolic profiles contain mechanistic information which could help identify biomarkers and therapeutic targets for ovarian tumours.
大多数卵巢肿瘤是上皮类型的，可进一步细分为良性、交界性或恶性。上皮性肿瘤通常有充满囊液的囊腔，由于囊液与肿瘤细胞 proximity 接近，其代谢谱反映了肿瘤细胞的代谢活性。采用基于1H-NMR光谱的代谢组学方法对来自良性、交界性和恶性卵巢肿瘤的卵巢囊液样本（n = 23）的代谢谱进行表征，以便更深入地了解卵巢肿瘤和癌症的发展。分析显示，与恶性肿瘤相比，良性肿瘤中柠檬酸盐升高，而与非恶性肿瘤相比，恶性肿瘤中氨基酸赖氨酸升高，两者均在5%的显著性水平。从良性样本到交界性样本再到恶性样本，胆碱和乳酸水平也逐渐升高。最后，仅在一部分恶性肿瘤中检测到次黄嘌呤。这项代谢组学研究表明，卵巢囊液样本有潜力用于区分不同类型的卵巢上皮性肿瘤。此外，各自的代谢谱包含有助于识别卵巢肿瘤生物标志物和治疗靶点的机制信息。
