Yan Wenwen, Wen Siwan, Wang Lemin, Duan Qianglin, Ding Lin
Department of Internal Medicine, Division of Cardiology, Tongji Hospital, Tongji University School of Medicine Shanghai 200065, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):18082-9. eCollection 2015.
To investigate the differential gene expression of cytokines and compare their impacts on the immune functions among the acute myocardial infarction patients (AMI), the stable angina patients (SA) and the controls.
20 patients with AMI, 20 patients with SA and 20 healthy volunteers were recruited into the study. Whole human genome microarray analysis was used to detect the gene expression differences in interferons, interleukins, chemokines, tumor necrosis factors and associated receptors in peripheral blood mononuclear cells (PBMCs) among three groups.
Compared with SA patients and the controls respectively, in AMI patients, IFNα2, IFNαR1, IFNαR2, IFNγR1, IFNγR2, L1β, IL16, IL18, Cxcl1, Cxcl2, Cxcl6, CxcR2, CxcR4, LIGHT, TNFR1, LT-βR, CD137, TRAILR, and TWEAKR mRNA expressions were significantly up-regulated (P<0.05), while Ccl5, Ccl24, Ccl28, CcR5, TWEAK, CD40, CD27, and BAFFR mRNA expressions were significantly down-regulated (P<0.05). But, there was no significant difference in cytokine expression between the SA patients and the controls.
In AMI patients, mRNA expression levels of cytokines were imbalanced, indicating the dysfunction of the immune system. Together with no significant change of cytokines was observed between the SA and controls, showing the different cytokine related immune activity in the AMI and SA patients.
研究急性心肌梗死患者（AMI）、稳定型心绞痛患者（SA）及对照组细胞因子的差异基因表达，并比较它们对免疫功能的影响。
招募20例AMI患者、20例SA患者和20名健康志愿者纳入研究。采用全人类基因组微阵列分析检测三组外周血单个核细胞（PBMCs）中干扰素、白细胞介素、趋化因子、肿瘤坏死因子及相关受体的基因表达差异。
与SA患者和对照组相比，AMI患者中IFNα2、IFNαR1、IFNαR2、IFNγR1、IFNγR2、IL1β、IL16、IL18、Cxcl1、Cxcl2、Cxcl6、CxcR2、CxcR4、LIGHT、TNFR1、LT-βR、CD137、TRAILR和TWEAKR的mRNA表达显著上调（P<0.05），而Ccl5、Ccl24、Ccl28、CcR5、TWEAK、CD40、CD27和BAFFR的mRNA表达显著下调（P<0.05）。但是，SA患者与对照组之间细胞因子表达无显著差异。
AMI患者细胞因子的mRNA表达水平失衡，提示免疫系统功能紊乱。SA患者与对照组之间细胞因子无显著变化，表明AMI患者和SA患者细胞因子相关免疫活性不同。
