急性冠脉综合征患者可溶性和膜结合 CD137 水平升高。
Enhanced levels of soluble and membrane-bound CD137 levels in patients with acute coronary syndromes.
机构信息
Department of Social Medicine and Health Management, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Clin Chim Acta. 2010 Mar;411(5-6):406-10. doi: 10.1016/j.cca.2009.12.011. Epub 2009 Dec 22.
BACKGROUND
Increasing evidence shows that costimulatory molecules of the tumor necrosis factor superfamily such as CD40/CD40 ligand and OX40/OX40 ligand have been implicated in atherosclerosis. We investigated whether the expression levels of the tumor necrosis factor superfamily members CD137 in serum and membrane-bound were related to acute coronary syndromes (ACS).
METHODS
Thirty normal controls and 210 patients, including 70 with stable angina (SA), 80 with unstable angina (UA), and 60 with acute myocardial infarction (AMI), were enrolled in our study. The expression of CD137 in peripheral monocytes was analyzed by flow cytometry. Serum soluble CD137 (sCD137) and C-reactive protein levels were measured by commercially available ELISA.
RESULTS
The expression of CD137 in peripheral monocytes in patients with UA [14.2+/-3.5 mean fluorescence intensity (MFI)] and AMI (15.1+/-4.4MFI) was significantly higher than those in patients with SA (6.5+/-2.4MFI) and controls (7.1+/-3.5MFI). sCD137 in patients with UA (16.7+/-4.9 ng/ml) and AMI (19.1+/-4.3 ng/ml) were significantly higher than those in patients with SA (3.4+/-1.4 ng/ml) and controls (3.9+/-1. 3 ng/ml) (p<0.001). C-reactive protein level in serum in patients with UA (13.8+/-3.3 ng/ml) and AMI (15.5+/-4.7 ng/ml) were also higher than those in patients with SA (1.4+/-0.4 ng/ml) and controls (1.3+/-0.3ng/ml). It was interesting that percutaneous transluminal coronary angioplasty (PTCA) induced a marked rise in sCD137 levels in SA patients, while CD137 expression in peripheral monocytes showed no difference between SA patients with PTCA before and after. A positive correlation was found between sCD137 and serum C-reactive protein levels (r=0.681; p<0.0001).
CONCLUSION
Patients with ACS showed increased soluble and membrane-bound CD137 expression. sCD137 level showed a significantly positive correlation with CRP level in patients with ACS. The relation between sCD137 and ACS needs further researches.
背景
越来越多的证据表明肿瘤坏死因子超家族的共刺激分子，如 CD40/CD40L 和 OX40/OX40L，与动脉粥样硬化有关。我们研究了血清和膜结合的肿瘤坏死因子超家族成员 CD137 的表达水平是否与急性冠状动脉综合征（ACS）有关。
方法
我们纳入了 30 名正常对照者和 210 名患者，包括 70 名稳定性心绞痛（SA）患者、80 名不稳定型心绞痛（UA）患者和 60 名急性心肌梗死（AMI）患者。通过流式细胞术分析外周血单核细胞中 CD137 的表达。通过商业上可获得的 ELISA 测量血清可溶性 CD137（sCD137）和 C 反应蛋白水平。
结果
UA 患者[14.2+/-3.5 平均荧光强度（MFI）]和 AMI 患者[15.1+/-4.4MFI]外周血单核细胞中 CD137 的表达明显高于 SA 患者[6.5+/-2.4MFI]和对照组[7.1+/-3.5MFI]。UA 患者的 sCD137[16.7+/-4.9ng/ml]和 AMI 患者[19.1+/-4.3ng/ml]明显高于 SA 患者[3.4+/-1.4ng/ml]和对照组[3.9+/-1.3ng/ml]（p<0.001）。UA 患者的血清 C 反应蛋白水平[13.8+/-3.3ng/ml]和 AMI 患者[15.5+/-4.7ng/ml]也高于 SA 患者[1.4+/-0.4ng/ml]和对照组[1.3+/-0.3ng/ml]。有趣的是，经皮腔内冠状动脉成形术（PTCA）可使 SA 患者的 sCD137 水平明显升高，而 SA 患者 PTCA 前后外周血单核细胞中 CD137 的表达无差异。sCD137 与血清 C 反应蛋白水平呈正相关（r=0.681；p<0.0001）。
结论
ACS 患者可溶性和膜结合 CD137 的表达增加。ACS 患者 sCD137 水平与 CRP 水平呈显著正相关。sCD137 与 ACS 的关系需要进一步研究。
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