Ren Jie, Lou Meiqing, Shi Jinlong, Xue Yajun, Cui Daming
Department of Neurosurgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine Shanghai, China.
Department of Neurosurgery, Shanghai First People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):18090-8. eCollection 2015.
Glioma is the most common form of primary brain tumor. Increasing evidence show that IDH1 gene mutation is implicated in glioma. However, the mechanism involved in the progression of glioma remains unclear until now. In the study reported here, we used gene chip to identifying the genes regulated with IDH mutanted at R132. The results showed that IDH1-mutant leads to 1255 up-regulated genes and 1862 down-regulated genes in U87 cell lines. Meanwhile, GO and gene-network was performed and shown IDH1-mutant mainly affect small molecule metabolic process, mitotic cell cycle and apoptosis. This result will lay a foundation for further study of IDH1 gene function in the future.
神经胶质瘤是原发性脑肿瘤最常见的形式。越来越多的证据表明，异柠檬酸脱氢酶1（IDH1）基因突变与神经胶质瘤有关。然而，迄今为止，神经胶质瘤进展所涉及的机制仍不清楚。在本文报道的研究中，我们使用基因芯片来鉴定受R132位点IDH突变调控的基因。结果显示，在U87细胞系中，IDH1突变导致1255个基因上调和1862个基因下调。同时，进行了基因本体（GO）和基因网络分析，结果表明IDH1突变主要影响小分子代谢过程、有丝分裂细胞周期和细胞凋亡。这一结果将为未来进一步研究IDH1基因功能奠定基础。
