Guo Dan, Li Ziqiong, Jia Bei, Che Xiaoqiong, Song Tianshuang, Huang Wenxiang
Chongqing Key Laboratory of Infectious Diseases and Parasitic Diseases, The First Affiliated Hospital of Chongqing Medical University No. 1 Youyi Road, Yu Zhong District, Chongqing 400016, China.
Int J Clin Exp Med. 2015 Oct 15;8(10):19454-9. eCollection 2015.
Besides being highly infectious, Hepatitis B virus (HBV) is a major cause of liver disease worldwide. In hospital settings, it is easy for the environment and quilts to be contaminated by HBV patient blood and body fluids. Therefore, HBV can be transmitted to other patients via contaminated environmental surfaces or quilts, resulting in an HBV nosocomial infection. Formaldehyde and ozone are commonly used disinfectants that may influence this infectious situation.
To investigate the clinical effectiveness of formaldehyde and gaseous ozone for the terminal cleaning of hospital quilts contaminated by HBV.
Thin cloth and thick cotton soaked with the serum from high HBV copy number patients were prepared and disinfected using formaldehyde fumigation and gaseous ozone at different times. The copy numbers of HBV DNA in the HBV-contaminated cloth and cotton samples were measured quantitatively with fluorescent quantitative polymerase chain reaction (PCR).
When gaseous ozone was used to disinfect HBV-contaminated quilts for 23 minutes (min), 36 min, 49 min, and 90 min, the HBV DNA copy number displayed no significant decrease compared with the copy number before disinfection (P > 0.05). In comparison, the copy number of the HBV DNA in the cloth group decreased significantly (P < 0.05) after formaldehyde fumigation disinfection for 1 hour (h), and there was no difference when longer times and increased concentrations were used. In the thick cotton group, there was also a significant decrease (P < 0.05) of the HBV DNA copy numbers, but the decrease was not as dramatic. In addition, in this group, the disinfection effect observed at 4 h was the strongest.
The application of ozone to disinfect HBV-contaminated hospital quilts possibly has no effect, whereas, formaldehyde oxide fumigation effectively reduced HBV copy numbers.
乙型肝炎病毒（HBV）除具有高度传染性外，还是全球肝病的主要病因。在医院环境中，环境和被褥很容易被乙肝患者的血液和体液污染。因此，HBV可通过被污染的环境表面或被褥传播给其他患者，导致医院感染HBV。甲醛和臭氧是常用的消毒剂，可能会影响这种感染情况。
探讨甲醛和气态臭氧对医院被HBV污染被褥进行终末消毒的临床效果。
制备用高HBV拷贝数患者血清浸泡的薄布和厚棉布，在不同时间分别用甲醛熏蒸和气态臭氧进行消毒。采用荧光定量聚合酶链反应（PCR）对被HBV污染的布和棉样本中的HBV DNA拷贝数进行定量测定。
使用气态臭氧对被HBV污染的被褥消毒23分钟（min）、36 min、49 min和90 min时，HBV DNA拷贝数与消毒前相比无显著下降（P>0.05）。相比之下，布组经甲醛熏蒸消毒1小时（h）后，HBV DNA拷贝数显著下降（P<0.05），延长时间和增加浓度时无差异。厚棉布组HBV DNA拷贝数也显著下降（P<0.05），但下降幅度没有那么大。此外，在该组中，4小时时观察到的消毒效果最强。
臭氧用于消毒被HBV污染的医院被褥可能无效，而甲醛熏蒸能有效降低HBV拷贝数。
