Eroglu Cafer, Zivalioglu Muammer, Esen Saban, Sunbul Mustafa, Leblebicioglu Hakan
Department of Clinical Microbiology and Infectious Diseases, School of Medicine, Ondokuz Mayis University, Samsun 55139, Turkey.
Hepat Mon. 2010 Winter;10(1):22-5. Epub 2010 Mar 1.
Hepatitis B virus (HBV) infection ranks among the most devastating health problems in the world.The most probable transmission routes of HBV are blood contact, sexual, and horizontal transfer. Other sources of HBV transmission are razor sharing, beauty treatments, tattooing, piercing, and manicures and other chiropody treatments.Many infections have been reported in South-East Asia, where barbers commonly share and reuse razors. Detection of HBV DNA in contaminated devices such as razor blades is important in the demonstration of transmission routes and indirect estimation of HBV prevalence in specific subpopulations such as barbershop clientele. Therefore, we aimed to detect the presence of HBV contamination on razor blades by nucleic acid testing.
Used razor blades (n = 151) were purchased from different barber's shops. Used razor blades purchased from chronic HBV patients (n = 8) were included as a positive control. The amplification and detection of HBV DNA was carried out by a semi-nested PCR method in a thermal cycler.
The presence of HBV DNA was found in 10 (6.6%) used razor-blade samples by the detection of a specific positive band with agarose gel electrophoresis.
In conclusion, used razor blades may be contaminated with HBV, and the practice of sharing used razor blades may pose a risk of transmission. Nucleic acid detection methods involving PCR can be used to detect HBV contamination of razor blades. HBV control and prevention programs should educate barbers about the importance of contagious diseases, proper sterilization techniques, and avoiding reuse and sharing of contaminated equipment and supplies such as razor blades. As an infection control measure, prohibition of razor reuse can reduce the spread of HBV infection in rural areas, where the practice is often common at barbershops.
乙型肝炎病毒（HBV）感染是世界上最具破坏性的健康问题之一。HBV最可能的传播途径是血液接触、性传播和水平传播。HBV的其他传播源包括共用剃须刀、美容治疗、纹身、穿孔以及修甲和其他足部护理治疗。东南亚地区报告了许多感染病例，那里的理发师通常共用和重复使用剃须刀。在诸如剃须刀片等受污染器械中检测HBV DNA对于证明传播途径以及间接估计特定亚人群（如理发店顾客）中的HBV流行率很重要。因此，我们旨在通过核酸检测来检测剃须刀片上HBV污染的存在情况。
从不同理发店购买用过的剃须刀片（n = 151）。从慢性HBV患者处购买的用过的剃须刀片（n = 8）作为阳性对照。通过半巢式PCR方法在热循环仪中进行HBV DNA的扩增和检测。
通过琼脂糖凝胶电泳检测到特定阳性条带，在10份（6.6%）用过的剃须刀片样本中发现了HBV DNA的存在。
总之，用过的剃须刀片可能被HBV污染，共用用过的剃须刀片的做法可能带来传播风险。涉及PCR的核酸检测方法可用于检测剃须刀片的HBV污染情况。HBV控制和预防计划应向理发师宣传传染病的重要性、正确的消毒技术以及避免重复使用和共享受污染的设备和用品（如剃须刀片）。作为一种感染控制措施，禁止重复使用剃须刀可减少农村地区HBV感染的传播，在农村地区理发店中这种做法通常很常见。
