Chai Ok Hee, Song Chang Ho
Department of Anatomy, Chonbuk National University Medical School and Institute for Medical Science, Chonbuk National University, Jeonju, Korea.
Anat Cell Biol. 2015 Dec;48(4):225-34. doi: 10.5115/acb.2015.48.4.225. Epub 2015 Dec 21.
Mast cells are known as effector cells of IgE-mediated allergic responses, but role of mast cells in contact hypersensitivity (CHS) has been considered controversial. In this study, we investigated role of mast cell in trimellitic anhydride (TMA)-induced CHS. The mice were sensitized to TMA on the back and repeatedly challenged with TMA on the left ear at 1-week intervals. The ear after challenge showed biphasic responses. The repetition of TMA challenge shifted in time course of ear response and enlarged the extent of early and late phase reactions in proportion to the frequency of TMA challenges in C57BL/6 mice. In late phase reaction, peak of ear response by single challenge showed at 24 hours after challenge, but the peak by repeat challenges at 8 hours after the last challenge. Number of mast cells and eosinophils per unit area increased in proportion to frequency of TMA challenges. However, mast cell-deficient WBB6F1/J-Kit(W)/Kit(W-v) mice developed the late phase reaction without the early phase reaction. The repetition of TMA challenge shifted in time course of ear response and enlarged the extent of ear response and the infiltration of eosinophils. The magnitude of these responses observed according to the frequency of the TMA challenge in mast cell-deficient WBB6F1/J-Kit(W)/Kit(W-v) mice was significantly lower than that in C57BL/6 mice. Also TMA elicited mast cell degranulation and histamine release from rat peritoneal mast cells in a concentration-dependent manner. Conclusively, TMA induces the early and late phase reactions in CHS, and mast cells may be required for TMA-induced CHS.
肥大细胞被认为是IgE介导的过敏反应的效应细胞，但肥大细胞在接触性超敏反应（CHS）中的作用一直存在争议。在本研究中，我们调查了肥大细胞在偏苯三酸酐（TMA）诱导的CHS中的作用。将小鼠背部致敏于TMA，并每隔1周用TMA反复攻击左耳。攻击后的耳朵表现出双相反应。在C57BL/6小鼠中，TMA攻击的重复改变了耳部反应的时间进程，并按TMA攻击频率成比例地扩大了早期和晚期反应的程度。在晚期反应中，单次攻击耳部反应的峰值出现在攻击后24小时，但重复攻击后的峰值出现在最后一次攻击后8小时。单位面积内肥大细胞和嗜酸性粒细胞的数量与TMA攻击频率成比例增加。然而，肥大细胞缺陷的WBB6F1/J-Kit（W）/Kit（W-v）小鼠出现了晚期反应，而没有早期反应。TMA攻击的重复改变了耳部反应的时间进程，扩大了耳部反应的程度和嗜酸性粒细胞的浸润。在肥大细胞缺陷的WBB6F1/J-Kit（W）/Kit（W-v）小鼠中，根据TMA攻击频率观察到的这些反应的强度明显低于C57BL/6小鼠。此外，TMA以浓度依赖性方式引起大鼠腹膜肥大细胞的脱颗粒和组胺释放。总之，TMA诱导CHS中的早期和晚期反应，肥大细胞可能是TMA诱导的CHS所必需的。
