Moussé Wassiyath, Sina Haziz, Baba-Moussa Farid, Noumavo Pacôme A, Agbodjato Nadège A, Adjanohoun Adolphe, Baba-Moussa Lamine
Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
Laboratoire de Microbiologie et de Technologie Alimentaire, FAST, Université d'Abomey-Calavi, ISBA-Champ de foire, 01 BP 526 Cotonou, Benin.
Biomed Res Int. 2015;2015:286473. doi: 10.1155/2015/286473. Epub 2015 Dec 7.
The present study aimed at biochemical and molecular characterization of Escherichia coli strains isolated from horticultural products and irrigation water of Cotonou. The samples were collected from 12 market gardeners of 4 different sites. Rapid' E. coli medium was used for identification of E. coli strains and the antimicrobial susceptibility was performed by the agar disk diffusion method. The β-lactamases production was sought by the liquid acidimetric method. The genes coding for β-lactamases and toxins were identified by PCR method. The results revealed that about 34.95% of the analyzed samples were contaminated by E. coli. Cabbages were the most contaminated by E. coli (28.26%) in dry season. All isolated strains were resistant to amoxicillin. The penicillinase producing E. coli carried blaTEM (67.50%), blaSHV (10%), and blaCTX-M (22.50%) genes. The study revealed that the resistance genes such as SLTI (35.71%), SLTII (35.71%), ETEC (7.15%), and VTEC (21.43%) were carried. Openly to the found results and considering the importance of horticultural products in Beninese food habits, it is important to put several strategies aiming at a sanitary security by surveillance and sensitization of all the actors on the risks of some practices.
本研究旨在对从科托努的园艺产品和灌溉水中分离出的大肠杆菌菌株进行生化和分子特征分析。样本采集自4个不同地点的12位菜农。使用快速大肠杆菌培养基鉴定大肠杆菌菌株，并通过琼脂纸片扩散法进行药敏试验。采用液体酸度法检测β-内酰胺酶的产生情况。通过PCR方法鉴定编码β-内酰胺酶和毒素的基因。结果显示，约34.95%的分析样本被大肠杆菌污染。旱季时，卷心菜受大肠杆菌污染最为严重（28.26%）。所有分离菌株均对阿莫西林耐药。产青霉素酶的大肠杆菌携带blaTEM（67.50%）、blaSHV（10%）和blaCTX-M（22.50%）基因。研究表明，这些菌株携带SLTI（35.71%）、SLTII（35.71%）、ETEC（7.15%）和VTEC（21.43%）等耐药基因。鉴于这些研究结果，并考虑到园艺产品在贝宁饮食习惯中的重要性，通过对所有相关人员进行风险监测和宣传来实施多项旨在保障卫生安全的策略十分重要。
