Center for Food Safety and Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30269; email:
International Center for Food Industry Excellence, Department of Animal and Food Sciences, Texas Tech University, Lubbock, Texas 79409.
Annu Rev Food Sci Technol. 2016;7:353-74. doi: 10.1146/annurev-food-041715-033259. Epub 2016 Jan 11.
As we are approaching the twentieth anniversary of PulseNet, a network of public health and regulatory laboratories that has changed the landscape of foodborne illness surveillance through molecular subtyping, public health microbiology is undergoing another transformation brought about by so-called next-generation sequencing (NGS) technologies that have made whole-genome sequencing (WGS) of foodborne bacterial pathogens a realistic and superior alternative to traditional subtyping methods. Routine, real-time, and widespread application of WGS in food safety and public health is on the horizon. Technological, operational, and policy challenges are still present and being addressed by an international and multidisciplinary community of researchers, public health practitioners, and other stakeholders.
随着脉冲网络（PulseNet）迎来成立二十周年，该网络由公共卫生和监管实验室组成，通过分子分型改变了食源性疾病监测的格局，公共卫生微生物学正在经历另一场变革，这是由所谓的下一代测序（NGS）技术带来的，这些技术使得对食源性细菌病原体进行全基因组测序（WGS）成为传统分型方法的一种现实且优越的替代方法。WGS 在食品安全和公共卫生方面的常规、实时和广泛应用即将实现。技术、运营和政策挑战仍然存在，一个由研究人员、公共卫生从业者和其他利益相关者组成的国际多学科社区正在解决这些挑战。
