Chung H J, Chung C P, Son S H, Nisengard R J
Department of Periodontology, College of Dentistry, Chonnam National University, Kwang Ju, Korea.
J Periodontol. 1989 Sep;60(9):506-11. doi: 10.1902/jop.1989.60.9.506.
Actinobacillus actinomycetemcomitans is though to play an important role in the pathogenesis of localized juvenile periodontitis (LJP). Preliminary data suggested that the serotype distribution of A. actinomycetemcomitans in Korea and the United States differ. This study evaluated A. actinomycetemcomitans prevalence, serotype distribution, and leukotoxicity in Korean LJP patients by culture, enzyme-linked immunosorbent assay, indirect immunofluorescence, and lactate dehydrogenase release from polymorphonuclear leukocytes exposed to A. actinomycetemcomitans. A. actinomycetemcomitans occurred in 75% of LJP lesions and 6% of normal sites with approximately equal distribution of serotype a, b, and c. Single serotypes were isolated from nine patients while three patients harbored two serotypes either in the same or different disease sites. A. actinomycetemcomitans leukotoxicity occurred in 22% isolates with a 69% prevalence. Individual sites harbored both leukotoxic and non-leukotoxic strains with no serotype association. The distribution of serotypes and leukotoxic strains of A. actinomycetemcomitans in Korean LJP patients differed from those reported in the United States. This suggests that serotype b may not be more important in the pathogenesis of LJP.
伴放线放线杆菌被认为在局限性青少年牙周炎（LJP）的发病机制中起重要作用。初步数据表明，韩国和美国的伴放线放线杆菌血清型分布有所不同。本研究通过培养、酶联免疫吸附测定、间接免疫荧光以及暴露于伴放线放线杆菌的多形核白细胞释放乳酸脱氢酶，评估了韩国LJP患者中伴放线放线杆菌的患病率、血清型分布和白细胞毒性。伴放线放线杆菌在75%的LJP病变和6%的正常部位出现，血清型a、b和c分布大致相等。从9名患者中分离出单一血清型，而3名患者在同一或不同疾病部位携带两种血清型。22%的分离株出现伴放线放线杆菌白细胞毒性，患病率为69%。个别部位同时存在白细胞毒性和非白细胞毒性菌株，与血清型无关。韩国LJP患者中伴放线放线杆菌的血清型和白细胞毒性菌株分布与美国报道的不同。这表明血清型b在LJP发病机制中可能并非更为重要。
